An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

Disclosed is a photosynthetic culture production device including at least one photo-bioreactor chamber having a supply/discharge unit, and including: an aqueous liquid containing a photosynthetic culture; at least one unit for supplying and discharging fluids from the chamber interacting with a management system; at least one light distributor including at least one first wall arranged so as to receive the light at a proximal end, at least one second wall arranged so as to emit at least part of the received light, and a sealed cavity defined by the at least one first wall and the at least one second wall, part of the emitting wall being immersed in the aqueous liquid containing the photosynthetic culture; at least one fluid partially filling the sealed cavity; and a cover, limiting evaporation. The cover has at least one opening, keeping the at least one light distributor stationary in the chamber.

A method for displacing a fluid and simultaneously gas enriching a liquid cell culture medium with a gas. The method includes injecting a controlled volume of a gas or gas mixture into a one chamber by using a gas flow controller, the injection taking place through a gas inlet into a volume of liquid. This injection produces bubbling and agitation of the volume of liquid; a build-up of gas or gas mixture due to buoyancy in a hermetic space formed by the volume of liquid and the chamber, and a pressure increase in the chamber until a sufficient controlled pressure is reached of less than or equal to 10 bar. This increase displaces the volume of liquid by a fluid outlet connecting the volume of liquid to the exterior of the chamber. Also provided are a device implementing the method and a cell culture system in a multi-well culture plate.

A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

Container for sample preparation or processing, such as biomass culturing or processing, and optionally sample purification. In certain embodiments, the reactor is a bioreactor that includes a stirred cell device that simulates a tangential flow filter to reduce or eliminate clogging that can be caused by the solids generated. In certain embodiments, the solids comprise a precipitate or floc or beads, such as one that includes a polymer that binds the biomolecule(s) of interest, and impurities. In its method aspects, embodiments disclosed herein include purification and isolation of biomolecules of interest derived from cell culture fluids. The methods include carrying out sample preparation or processing in a container, culturing a biomass; generating solids by precipitating or flocculating a biomolecule of interest from the cultured broth; preventing the solids from settling in the container by agitation; and purification, such as by eluting the biomolecule of interest and filtering the same.

Gas-utilizing microorganisms are stably cultured regardless of variations in a supply flow rate of a substrate gas. Gas-utilizing microorganisms 9 are cultured in a culture solution 2 in a culture tank 10. A substrate gas containing CO and H.sub.2 or the like is supplied to the culture tank 10 and is dissolved in the culture solution 2. When a supply flow rate of the substrate gas or predetermined constituents of the substrate gas to the culture tank 10 becomes a predetermined value or lower, a culture solution 2a is rapidly discharged from the culture tank 10.

A method for the continuous flow synthesis of (R)-4-halo-3-hydroxy-butyrate using a micro-reaction system. The micro-reaction system includes a micro-mixer, a certain number of micro-reaction units that are successively connected in series, a pH regulating system and a back pressure valve. The micro-reaction unit is composed of a micro-channel reactor and a pH regulator that are sequentially connected with each other. A substrate solution containing halogenated acetoacetate and a biocatalyst solution are simultaneously pumped into the micro-reaction system to enable continuous flow biocatalytic asymmetric reduction reaction of the halogenated acetoacetate to obtain the target product (R)-4-halo-3-hydroxy-butyrate.

The present invention is directed to a multi-organ-chip device comprising a base layer; an organ layer arranged on the base layer; an antra layer arranged on the organ layer; and an actuator layer; wherein the base layer is configured to provide a solid support for the further layers; the organ layer is configured to comprise a multiplicity of individual organ equivalents, each organ equivalent comprising one or more organ growth sections, each of the organ growth sections being configured to comprise an organoid cavity for housing at least one organoid of an organ and to comprise a micro-inlet and a micro-outlet for fluid communication between the organoid cavity of the organ growth section and a self-contained circulation system, wherein the organ layer comprises at least one organ equivalent configured to represent the organs lung, small intestine, spleen, pancreas, liver, kidney and bone marrow, respectively, and a self-contained circulation system configured to be in direct fluid communication with the organ growth sections of the organ layer via the micro inlets and outlets of the organ growth sections; the antra layer is configured to comprise a multiplicity of cavities and tubes arranged to be in fluid communication with selected organ equivalents or organ growth sections in order to allow for exchange of fluids between cavities and organ growth sections; and the actuator layer is configured to comprise a multiplicity of actuators arranged and configured to regulate a pressure force applied on a selected organ equivalent, the self-contained circulation system and/or part thereof.

A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

An exemplary embodiment of the present invention provides an apparatus for producing fermented soybean meal, which produces fermented soybean meal for monogastric animals and ruminant selectively or together. An apparatus for producing fermented soybean meal according to an exemplary embodiment of the present invention includes: a solid-liquid separating part, which mixes raw material soybean meal and an extraction solvent and extracts the soybean meal, and separately produces a remaining soybean meal and a soybean meal extract; a lactic acid bacteria culturing part, which produces the lactic acid bacteria by putting inoculum into the soybean meal extract, and supplies the lactic acid bacteria to the solid-liquid separating part; a solid substrate fermenting part, which is selectively supplied with and mixes at least two of the remaining soybean meal supplied from the solid-liquid separating part, the raw material soybean meal supplied through bypass, and lactic acid bacteria supplied from the lactic acid bacteria culturing part to produce a mixed material, and solid-substrate ferments the mixed material to produce primary solid substrate fermented soybean meal; and a drier, which dries the primary solid substrate fermented soybean meal supplied from the solid substrate fermenting part to produce secondary solid substrate fermented soybean meal.