Embodiments of the present disclosure provide a biological sample imaging device, including an incubator with an observation window provided on one side; a biological sample cultivation module provided inside the incubator; an imaging module provided outside the incubator for collecting images of a biological sample cultured in the biological sample cultivation module through the observation window, so that the growth process can be dynamically captured, and the environment in the incubator can be conveniently controlled to adapt to various scientific research scenarios. In addition, the isolation inside and outside the incubator can reduce the interference of the imaging module on the biological sample.

A system for aerobic fermentation includes a vessel, an aeration system including a gas sparger fluidly coupled to the vessel to introduce a compressed gas to an internal volume of the vessel, and a recirculation loop fluidly coupled to an outlet of the vessel. The recirculation loop includes an eductor fluidly coupled to an oxygen-containing gas source, a static mixer downstream of the eductor, a heat exchanger downstream of the eductor, and a distributor downstream of the heat exchanger. The distributor is fluidly coupled to the vessel. The aeration system provides mixing and oxygen mass transfer to the fermentation composition in the vessel. The fermentation composition passes through the eductor, static mixer, heat exchanger, and distributor of the recirculation loop, and back into the vessel. Oxygen is transferred from an oxygen containing gas to the fermentation composition and heat is removed from the fermentation composition in the recirculation loop.

An anaerobic digestion system is provided. The system includes a biomass storage container and a cover positioned over the biomass storage container, where the cover is sealed at an outer edge of the anaerobic digester. Weights may be arranged on the surface of the cover, and the sealed edges may form a water collection region. Additional systems, including thermal management, gas processing, energy storage and recovery, and sensing can also be included.

The present invention provides a stem cell manufacturing system comprising: a pre-transfer cell solution sending channel 20 through which a solution containing cells flows; an inducer solution sending mechanism 21 which sends a pluripotency inducer into the pre-transfer cell solution sending channel 20; an inducer transfer apparatus 30 which is connected to the pre-transfer cell solution sending channel 20 and transfers the pluripotency inducer into the cells to produce cells harboring the inducer; a cell cluster production apparatus 40 which cultures the cells harboring the inducer to produce a plurality of cell clusters consisting of stem cells; and a packaging apparatus 100 which sequentially packages the plurality of cell clusters.

Tissue culture platforms that may be configured for tissue culture or biological cell culture, and methods for use thereof, are described. In general, the tissue culture platforms include multiple well chambers that are fluidically coupled by one or more channels Flow between the different well chambers is controlled via one or more selector valves, enabling a single tissue culture platform that can provide multiple integrated culture subsystems, multiple non-interacting culture subsystems, or combinations thereof.

An automated apparatus for preparing a liquid bioprocess solution includes at least one mixing chamber having a lower port and an upper port for fluid to enter the at least one mixing chamber, an array of tubing for fluid flow within the system, a plurality of valves provided within the tubing, and a mixing controller configured to cause the automated apparatus to perform a series of sequential mixing steps causing the preparation of the liquid bioprocess solution from a dry ingredient. The series of sequential mixing steps include opening a first valve associated with the lower port to provide fluid to the at least one mixing chamber through the lower port, and after a predetermined amount of elapsed time, closing the first valve and opening a second valve associated with the upper port to provide fluid to the at least one mixing chamber through the upper port.

A microfluidic device comprising a microfluidic network is described. The device comprises a base, a microfluidic channel and a cover, and the base comprises a diaphragm forming at least part of an inner surface of the microfluidic channel. The device finds use in methods for assessing mechanical strain induced in or by cells, such methods also being described.

A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

The invention relates to a bioreactor (100) which is designed for cultivating biological cells (1), comprising a container (10) configured to receive a cultivation medium (2), and a plurality of substrate filaments (20) which are arranged in the container (10) and are configured for a temporary adherent coupling of the biological cells (1) to the substrate filaments (20). The substrate filaments (20) are provided with a surface layer (21) which is switchable between an adherence state, in which the biological cells (1) can be QI coupled adherently to the surface layer (21), and a release state, in which the adherent coupling of the biological cells (1) to the surface layer (21) is reduced in comparison to the binding state. The invention also relates to a method for processing biological cells (1) in the bioreactor (100).

The present invention relates to the cultivation of microalgae for the production of biofuels. In this scenario, the present invention provides a vertical flow agitation system for microalgae culture tanks comprising: a source of energy generation (1); an energy storage device (2); a control system (3); an electric motor (4); at least one end of travel sensor (5); an agitation plate (6); a torque transmission system (7); and at least two lateral drive elements (8).