A cell culture system includes: a first pump; and a first cell culture container and a second cell culture container. The first cell culture container has a first culture medium circulation flow path. The second cell culture container has a second culture medium circulation flow path independent of the first culture medium circulation flow path. The first culture medium circulation flow path and the second culture medium circulation flow path are fluidly connected to the first pump.

The invention discloses a bioreactor with a vessel defining an inner volume, agitation means and at least one addition tube, wherein a delivery orifice in the addition tube is located within the inner volume and a check valve is arranged in proximity of the delivery orifice for allowing flow of a fluid in the direction from the addition tube into the inner volume of the vessel and blocking flow in the reverse direction.

A system for introducing a vector into includes a filter module defining an intra-capillary space and an extra-capillary space separated from the intra-capillary space by a porous membrane. The system also includes a pair of intra-capillary ports fluidly coupled to opposite ends of the intra-capillary space and each receiving a transduction media, cells, and a vector. The system also includes a pair of extra-capillary ports coupled to opposite ends of the extra-capillary space and in fluid-communication with a source of extra-capillary media and a waste container.

According to one broad aspect of this disclosure, a method is provided for producing polyhydroxyalkanoates (PHA) from organic waste. The method comprises homogenizing organic waste to obtain a feedstock that has 1:1 to 3:1 (w/w) water to organic waste ratio. The feedstock is inoculated with an inoculum of acidogenic fermentative bacteria in order to obtain an inoculated feedstock. The inoculated feedstock is incubated for 5 to 10 days, 3 to 10 days, optionally 7 days, optionally 3 days, to obtain a fermentation broth. The fermentation broth comprises volatile fatty acids (VFAs) and undigested organic waste. The fermentation broth is filtered with a filter with a pore size ranging from 0.2 μm to 500,000 NMWC to remove the acidogenic fermentative bacteria and undigested organic waste, to obtain a clarified broth comprising concentrated VFAs. The clarified broth and high-PHA producing bacteria are incubated to produce intracellular PHA granules in the high-PHA producing bacteria. PHA polymers are extracted from the intracellular PHA granules.

An apparatus for washing an array plate includes one or more dispensers and one or more aspirators that are distinct from the one or more dispensers. A respective dispenser of the one or more dispensers is configured to dispense a first liquid on the array plate, and a respective aspirator of the one or more aspirators is configured to aspirate liquid on the array plate. A method for washing particles is also disclosed.

A system and method of fluid delivery for providing a surface fluid pattern. The system includes a fluid delivery head for fluid flow therethrough. The fluid delivery head includes a fluid delivery surface having surface openings defined therein and arranged across the fluid delivery surface as a two-dimensional display. Some of the surface openings are grouped as a surface opening unit. The surface opening unit includes at least one aspiration opening through which fluid can be provided to the fluid delivery surface and at least one injection opening through which fluid can be moved away from the fluid delivery surface. The surface opening unit includes at least three surface openings positioned as a two-dimensional display and outwardly of at least one other surface opening.

A treatment apparatus for treating biological cell cultures, located in respective storage chambers of a carrier, includes a work deck with one or more bearing spots for bearing the carrier. Each of the bearing spots includes a bearing region corresponding to an area of the carrier. The treatment apparatus additionally includes nozzles for producing a gas flow in a space above the work deck. To avoid liquid droplets or cell material being transported from one storage chamber to another as a result of a horizontal gas flow above the carrier during the treatment, the nozzles are arranged outside of and along an edge of the bearing region or within the bearing region and form a gas curtain around the carrier or the respective storage chambers.

A process and system for producing an inoculum for downstream cell production is disclosed. The inoculum is produced in a perfusion bioreactor in which the nutrient media feed is increased as the biomass concentration increases within the bioreactor. A biomass sensor can be used to periodically or continuously monitor biomass concentration. This information can be fed to a controller for automatically increasing nutrient media feed rates in a manner that is directly proportional to producing an inoculum with an increase cell density. The process and system can also include an automated subsystem for maintaining constant volume levels within the perfusion bioreactor during the process.

A cell culture device (200) includes a cell culture vessel (22) for culturing cells, a factor container (81) for storing a factor, a reagent container (82) for storing a reagent for introducing the factor into the cells, and a flow path for transporting the factor and the reagent from the factor container (81) and the reagent container (82) to the cell culture vessel (22).

A device to aid in the production and regeneration of tissues and organs, standing alone, or on or inside of the human body, with a method of fabrication of tissues and organs and use of the device.