The present disclosure provides a method of producing uniformly sized organoids/multicellular spheroids using a microfluidic device having an array of microwells. The method involves several successive steps. First, a microfluidic device containing parallel rows of microwells that are connected with a supplying channel is filled with a wetting agent. The wetting agent is a liquid that is immiscible in water. For example, the wetting agent may be an organic liquid such as oil. In the next step, the agent in the supplying channel and the microwells is replaced with a suspension of cells in an aqueous solution that contains a precursor for a hydrogel. Next, the aqueous phase in the supplying channel is replaced with the agent, which leads to the formation of an array of droplets of cell suspension in the hydrogel precursor solution, which were compartmentalized in the wells. The droplets are then transformed into cell-laden hydrogels. Subsequently, the agent in the supplying channel is replaced with the cell culture medium continuously flowing through the microfluidic device and the cells within the hydrogels are transformed into multicellular spheroids.

This cell production device comprises: a cell production plate that includes a fluid circuit having a plurality of functional parts integrated therein; and a plurality of closed type connectors that connect the fluid circuit to an external space in a closed manner. The fluid circuit comprises, as the plurality of functional parts: a plurality of injection and discharge units capable of injecting or discharging a plurality of types of fluids into or out of the fluid circuit via the plurality of closed type connectors; a plurality of fluid reservoir units capable of storing the plurality of types of fluids to be injected or discharged; and a cell induction and culture unit that performs at least one of induction and culture of cells based on the stored plurality of types of fluids.

Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.

The present invention relates to a device for cultivating cells, in particular tissue, comprising a carrier plate unit which has a central axis of rotation, at least one access opening arranged proximally to the axis of rotation, at least one cultivation chamber arranged distally to the axis of rotation, and at least one channel connecting the access opening to the cultivation chamber, and also a method for cultivating cells in a device according to the invention and a method for producing the device according to the invention.

The present disclosure is drawn to microfluidic cellular membrane modification devices. In one example, a microfluidic cellular membrane modification device can include a microfluidic channel including a pumping portion and an electric field portion. An electrode pair can be positioned about the electric field portion. A bidirectional pump can be in fluid communication with the microfluidic channel at the pumping portion to move fluid backward and forward through the electric field portion.

The present disclosure relates to methods of preparing personalized blood vessels, useful for transplantation with improved host compatibility and reduced susceptibility to thrombosis. Also provided are personalized blood vessels produced by the methods and use thereof in surgery.

A fluid supply interface includes at least one supply coupling configuration, at least one discharge coupling configuration, at least one user coupling configuration, and a fluid conduit that connects the supply, discharge, and user coupling configurations to one another. A supply valve is capable of having fluid flow through it or is blocked for flow through it. A discharge valve is capable of having fluid flow through it or is blocked for flow through it. The supply valve is preloaded into a blocking position that prevents flow, and opens by means of a sufficiently large pressure difference between the two sides of the supply valve, against the preload force, for flow through in a direction from the supply coupling configuration toward the fluid conduit. The discharge valve likewise being preloaded into a closed position that prevents flow through it.

The present invention relates to an automatic culture device using a single-use closed system flow path for culturing a cell or a tissue, and realizes reduction in manufacturing cost and high integration property of a device. A cell culture system including an automatic culture device and an information processing device. The automatic culture device includes a plurality of types of closed system flow paths possible to be installed and removed, and a plurality of culture devices. The information processing device includes: an input device configured to receive, as an input, at least one piece of data selected from a group consisting of data of an identifier of a patient, data related to a transplantation method, data related to a type of cell, data related to the required number of cells, and data related to a treatment plan; an arithmetic device configured to select, based on the input data, from options of a cell culture method, the culture devices, and the closed system flow paths, the cell culture method, the culture device, and the closed system flow path to be used; and an output device configured to output a number of the closed system flow path to be used and a number of the culture device to be used.

Example embodiments include a cell culture apparatus, methods for cell cultivation by using the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus. The cell culture apparatus comprises a plurality of cell culture modules arranged adjacent to each other. Each cell culture module comprises two or more culture medium reservoirs connected by two or more flow channels that go through a basal chamber arranged under an apical chamber. The apical and basal chambers are separated by a porous membrane. The basal chamber has a bottom part arranged higher than a bottom part of each of the culture medium reservoirs. The culture model comprises further a cavity under at least the bottom part of the basal chamber and at least one means for capturing at least one image from a bottom and/or top of the at least one cell culture module.

A microphysiological platform described herein includes a fluidic synthesizer with a first fluid input selectively coupleable to a source of a first input fluid solution and a second fluid input selectively coupleable to a source of a second input fluid solution. The fluidic synthesizer further includes a fluid output. The microphysiological platform further includes a fluid addressing system with a fluid input fluidically coupled to the fluidic synthesizer fluid output. The fluid addressing system further includes a first fluid output and a second fluid output. The microphysiological platform further includes a first microphysiological device with a fluid input fluidically coupled to the first fluid output of the fluid addressing system and a second microphysiological device with a fluid input fluidically coupled to the second fluid output of the fluid addressing system.