A technology of 3D printing integration of hard materials and cells, a preparation of bone-repair functional module with osteogenic microenvironment, bone organoid method and the application of quick repair of bone defects are provided. A preparation method of biological microenvironmental factors as independent osteogenic factors is further provided. The present integrated 3D printing technology realizes 3D printing of cells and hard materials synchronously by adjusting the temperature, so as to build a real sense of biomimetic bone tissue, which can be customized according to the specific defects and clinical needs of patients. In the present bone-repair functional module, the cells have high survival rate and proliferation activity on the surface of hard materials, and realize osteogenic differentiation and mineralization; after implantation, it has the dual metabolic functions of bone formation and bone resorption, promoting vascular and neurogenesis, improving elastic modulus and reducing stress shielding.

A compound algae culture apparatus is provided, which includes a photobioreactor module, a growth regulating module, a circulation transfer module, and a circulation pipeline module. The photobioreactor module includes at least one photobioreactor unit, and the growth regulating module includes at least one growth tank unit. The photobioreactor unit includes a light-transmitting coiled pipe. The growth tank unit has a tank body, and a plurality of partitions are disposed in the tank body to divide an inside of the tank body for formation of a curved flow channel A culture fluid for culturing algae passes through the photobioreactor unit and enters the growth tank unit. A volume of the growth tank unit is larger than a volume of the photobioreactor unit, and a residence time of the culture fluid in the growth tank unit is greater than a residence time of the culture fluid in the photobioreactor unit.

An apparatus for sampling a cell culture includes a sampler for connecting to a bioreactor with a fixed bed including the cell culture. A releasably attached sample portion for positioning within or adjacent the fixed bed includes a substrate on which cells may be grown and then removed from the bioreactor to provide an indication of cell growth. A kit for sampling a cell culture bed of a bioreactor includes the sampler for sampling the cell culture bed with the releasably attached sample portion, along with a container adapted to detach the sample portion from the sampler. A method of obtaining a sample of a cell culture in a bed within a bioreactor includes positioning a sampler positioned at least partially in or adjacent to the bed to promote the growth of cells on a releasably attached portion of the sampler, withdrawing the sampler from the bioreactor, and detaching the portion of the sampler.

A 3D scaffold of a biocompatible polymer and colonized with biological cells is provided., The biological cells can be cultured to form a 3D cell culture construct that closely approximates a physiological architecture. A method for producing the 3D scaffold colonized with biological cells is also provided.

The invention provides devices, systems, and methods for 3D printing. The invention employs slurries of particles or a solute in a carrier fluid, which may be a liquid or gas, in printing. The use of a slurry is advantageous in allowing for printing in any orientation.

A method of manufacturing a microstructure comprises printing a positive mold structure, filling the positive mold structure with a second material to form an elastically deformable negative mold structure, filling the negative mold structure with a third material to form the microstructure, and releasing the microstructure from the negative mold structure. Advantageously, the negative mold structure can be stretched to facilitate the release of the microstructure. For example, the microstructure comprises a chamber with capped micropillars for the generation and/or analysis of muscle tissue.

A bioprocessing system comprising a series of processing stations for performing operations for bioprocessing is disclosed. The bioprocessing system includes an automated system comprising means for manipulating a fluid connection between a first container and a separable second container whereby to create an aseptic connection that enables a controlled transfer of fluid or cell material between the first container and the second container, wherein the means for manipulating a fluid connection is configured to create an aseptic connection that can be disconnected after the transfer of fluid or cell material is complete to enable a further such fluid connection to be manipulated between the first container and a separable third container, and means for controlling an automated sequence of operation of the processing stations.

A system and method for printing cells in a medium. A multi-dimensional printer, stably constructed of low-mass parts, can include a computer numerically controlled system that can enable motors driving delivery systems. The motors can include encoders that can enable achieving arbitrary resolution. The motors can drive ballscrews to enable linear motion of delivery systems, and the delivery systems can enable printing of a biological material in a pre-selected pattern in a petri dish. The petri dish can accommodate a medium such as a gel, and can further accommodate a vision system that can detect actual position and deflection of the delivery system needle. The printer can accommodate multiple delivery systems and therefore multiple needles of various sizes.

A network (100) for replacement of a living tissue, said network is a scaffold-free artificial hollow biological tissue network comprising a plurality of longitudinal multicellular aggregates (11) arranged in a plurality of bioprinted layers (22) which are located on top of one another, further comprising an inner surface (20) and an outer surface (21), wherein at least one of said bioprinted layers (22) is in shape of a planar closed loop such that a conduit for conveying fluids is defined, and said longitudinal multicellular aggregate (11) is a mixture of at least two cell types. Also a method for obtaining said longitudinal multicellular aggregate, and a further method for biomodeling and planning said network are proposed.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.