Techniques for printing living tissues and organs are provided. An example apparatus includes a printing platform, bioink printing module with at least one nozzle designed for bioink dosing, a gel-forming composition printing module containing a UV-module, and a nozzle for dosing gel-forming composition that starts polymerizing via UV radiation. A module is provided for relatively displacing the nozzles and/or the platform, in which the bioink printing module is separated from the gel-forming-composition printing module to prevent UV radiation from reaching the bioink printing module. The radiation is directed predominantly parallel to the printing platform to prevent UV radiation from reaching the biological tissues and/or organs being printed. A multi-functional device capable of combining various printing modes, providing a method of high-resolution printing of living tissues and organs based on UV-induced hydrogel polymerization, and a method of cell protection from UV radiation during the printing process, can be realized.

A system for buoyant particle processing includes: a reaction vessel, a stirring mechanism, a set of one or more pumps, and a filter. The system can additionally or alternatively include a set of pathways and/or any other suitable component(s). A method for buoyant particle processing includes: stirring the contents of a reaction vessel; washing a set of buoyant particles; and filtering the contents of the reaction vessel. Additionally or alternatively, the method can include any or all of: preprocessing the set of buoyant particles; adding a set of inputs to the reaction vessel; washing the set of buoyant particles; repeating one or more; and/or any other suitable process(es).

Provided is a cell-culturing apparatus including a culturing unit in which a culturing vessel is placed and an incubator in which a plurality of culturing units are placed in an interior thereof, and that can maintain the interior thereof at an environment that is appropriate for cell culturing, wherein the incubator includes openings that are provided for each of the plurality of culturing units and through which the culturing units can separately be moved into and out of the incubator, and closing means that are provided at the openings and that seals off the internal environment of the incubator from outside when the culturing unit is taken out of the incubator.

A stem cell manufacturing system for manufacturing stem cells from somatic cells includes: one or more closed production device(s) configured to produce stem cells from somatic cells; one or more drive device(s) configured to be connected with the production device(s) and drive the production device(s) in such a manner as to maintain the production device(s) in an environment suitable for producing stem cells; one or more cryopreservation device(s) configured to cryopreserve the produced stem cells; a first memory device configured to store whether or not somatic cells have been introduced to the production device(s), as a first state; a second memory device configured to store whether or not the production device(s) is/are connected with the drive device(s), as a second state; and a third memory device configured to store whether or not the produced stem cells can be placed in the cryopreservation device(s), as a third state.

The disclosure relates to methods, systems and compositions for physically manipulating a muscle tissue culture either mechanically, or manually, or both. Specifically, the disclosure relates to systems and methods of physically manipulating, either mechanically or manually, a resilient container of bioprinted tissue culture having non-random three dimensional cell structure by elongation, compression, torque and shear of the tissue culture.

In a method for the production and/or treatment of a three-dimensional object with a printing material which is dispensed at a target position in the form of discrete three-dimensional printing material elements, a metering device is moved to at least one reservoir in which a supply of printing material is kept and, by means of the metering device, printing material is picked up from that at least one reservoir. The metering device is transported to a target position defined in all three spatial dimensions and, at that target position, a metered quantity of printing material is applied to a substrate or to a three-dimensional object arranged thereon or being constructed thereon, in order to create a printing material element. The creation of a printing material element is repeated until the three-dimensional object has been fully constructed and/or treated. The use of a metering device for picking up, transporting and applying printing material makes it possible for virtually any desired printing materials to be processed and, accordingly, for objects to be produced and/or treated with virtually any desired printing materials.

A pre-programmed non-feedback continuous feeding method based on mass balance of the substrate in the bioreactor for use in culture growth and maintenance is provided. The disclosed method does not rely on instrument, probe or operator feedback. The method provides an efficient and effective alternative to bolus feeding.

An operation isolator forms an aseptic space. An incubator is connected to the operation isolator, in which cells are stored and cultured. A storage chamber stores articles used in the operation isolator. In order to carry articles from the outside into the storage chamber, a decontamination pass-box is provided. The storage chamber and the operation isolator are directly or indirectly connected to each other.

An assembly and method to precisely and concurrently control the concentration, release, and depletion of chemical and biochemical species in localized regions in a three-dimensional volume of material using spatially patterned light. The system includes a container for holding a material comprising photo-responsive substance; the container is mounted to a motorized rotation stage that rotates the container along the z-axis at a predetermined rate; and a projector configured to project radiations at predefined wavelengths along an r-axis into the container while the container is rotating, wherein the radiations from multiple angles intersect to form localized photoreactive regions within a volume of the material.

A bacteria sampling rod in the present disclosure comprises a rod body that has a comb teeth portion at the front end to contact with a culture dish through a user's operation similar to affixing a seal or making a rotation, catch and collect bacteria on the culture dish by the comb teeth portion, and transfer bacteria into a test vessel in which the bacteria solution is diluted for final analysis at a drug sensitivity test in an analyzer. The bacteria sampling rod can be installed on an automated machine properly for a full-automatic standardized operation as well as improved working performance and quality; the bacteria sampling rod with the advantage of an elastic buffering effect is free from the risk of a culture dish punctured during bacteria collection.