A transfection device suitable for delivery of various macrostructures (e.g., mitochondria, bacteria, liposomes) is described and uses mechanical force to thereby induce active endocytosis in a target cell. Contemplated devices are able to achieve high throughput of transfected cells that remain viable and are capable of producing colonies.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

A soft sphere transfer device, includes: a water tank into which soft spheres are introduced; a transfer rotation member rotatably disposed in the water tank and including a plurality of accommodation grooves formed along a peripheral edge of the transfer rotation member; and an injection device configured to inject a predetermined substance into the soft spheres by piercing a needle into each of the soft spheres transferred to a needle piercing part by rotation of the transfer rotation member, wherein the water tank includes a pair of side walls facing each other in a width direction, and wherein a narrow width portion having a width equal to or smaller than a diameter of the soft spheres is provided on at least one portion in the width direction among portions of the side walls corresponding to the needle piercing part.

In various embodiments, method and devices for delivering large cargos (e.g., organelles, chromosomes, bacteria, and the like) into cells are provided. In certain embodiments method of delivering a large cargo into eukaryotic cells, are provided that involve providing eukaryotic cells disposed on one side of a porous membrane; providing the cargo to be delivered in a solution disposed in a reservoir chamber on the opposite side of the porous membrane; and applying pressure to the reservoir chamber sufficient to pass the cargo through pores comprising said porous membrane wherein said cargo passes through cell membranes and into the cells.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

The present disclosure provides automated multi-module instruments, compositions and methods to increase the percentage of edited mammalian cells in a cell population when employing nucleic-acid guided editing.

An ultrasonic cellular stimulation device includes: an ultrasonic transducer for generating a vibration wave; an upper cap having a fixing portion disposed on a lateral surface thereof; and a lower cap having a first surface and a second surface, and including: a recess indenting the first surface; an abutment portion located at a bottom of the recess; and an opening formed in the second surface and being in communication with the recess. The upper cap is partially received in the recess. The ultrasonic transducer is clamped and fixed between the fixing portion and the first surface. A glass piece is disposed on the abutment portion and fixed between the upper cap and the lower cap, such that the vibration wave is conveyed from the first surface into the abutment portion and the glass piece and a uniform and controllable sound field is generated above the glass piece.