In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

Provided is a CellShot platform for the injection of a material into a flowing cell, in which a high-speed flow cytometer is connected to a 2D intracellular delivery injector, and a method of controlling the delivery of a material into a cell using the same. When a CellShot platform according to the present disclosure is used, a delivery material may be injected into flowing cells at high throughput and with excellent injection efficiency. In this regard, large molecules may also be efficiently delivered and excellent cell viability is exhibited after injection, which can be broadly applied to multiplex personalized cancer vaccine development as well as advanced cell research.

The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

Methods, tip assemblies and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition.

The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

A cell culture apparatus (1) of the present invention comprises: a culture vessel (2) in which a cell culture medium comprising at least one of culture components composed of a conjugate with a stimuli-responsive polymer is stored and cells are cultured in the medium; a stimulus-applying mechanism (4) that applies a predetermined stimulus to the conjugate so as to induce a predetermined change of the stimuli-responsive polymer in response to the stimulus; and, a separation mechanism (5) that separates at least a part of the medium components except for the conjugate from the cell culture medium, while leaving the conjugate in the cell culture medium, on the basis of a property change of the stimuli-responsive polymer.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.