A blade tip-provided micropipette holding apparatus has a tubular micropipette holder so constructed that a blade tip-provided micropipette to be inserted into a living cell can be mounted on a front-end portion thereof and a holder-holding device for holding the micropipette holder. The holder-holding device has a base member and a tubular rotating member which is rotatably held by the base member and into which the tubular micropipette holder can be penetrated. The tubular rotating member has holder position adjusting mechanisms each composed of a plurality of side holes formed on a side surface of the tubular rotating member at different positions of the side surface thereof in a circumferential direction thereof and holder position adjusting members each capable of entering into the tubular rotating member from the side holes and contacting an outer surface of the micropipette holder penetrating through the tubular rotating member.

Nanostraws and to methods of utilizing them in order to deliver biologically relevant molecules such as DNA, RNA, proteins etc., into non-adherent cells such as immune cells, embryos, plant cells, bacteria, yeast etc. The methods described herein are repeatedly capable of delivering biologically relevant cargo into non-adherent cells, with high cell viability, dosage control, unaffected proliferation or cellular development, and with high efficiency. Among other uses, these new delivery methods will allow to scale pre-clinical cell reprogramming techniques to clinical applications.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

In an illustrative embodiment, cell libraries created by rationally designed nucleic acids comprising sequences to facilitate nuclease-directed genome editing events are provided. The cell libraries include saturation mutagenesis cell libraries, promoter swap cell libraries, SNP swap cell libraries, terminator swap cell libraries, and combinations thereof.

A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.

Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.

In a first aspect of the present invention, a manipulation method of an organism is provided which includes: forming a gas-liquid interface, in which a restoring force acts against minute interface movement, in a flow channel or at an end part of the flow channel, with the end part immersed in a liquid in which an organism is immersed; and manipulating the organism by using the gas-liquid interface.

An object of the present invention is to provide a novel technique or method for controlling intracellular reaction that allows introduction of a specific factor into a large number of cells with simple and efficient operations, and the present invention provides a method for controlling intracellular reaction, the method including the steps of: bringing a needle-shaped body having an intracellularly introducible factor immobilized thereon into contact with a cell and inserting a part of the needle-shaped body into the cell to introduce the intracellularly introducible factor into the cell; and withdrawing the part of the needle-shaped body from the cell.

The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.