A stem cell production system provided with a preintroduction cell-feeding solution channel 20 through which a solution containing cells passes, an induction factor-feeding solution mechanism 21 for feeding a pluripotency induction factor to the preintroduction cell-feeding solution channel 20, a factor introduction device 30 connected to the preintroduction cell-feeding solution channel 20 for making cells with induction factor introduced by introducing the pluripotency induction factor into the cells, a cell mass-making device 40 for making multiple cell masses comprising stem cells by culturing the cells with induction factor introduced, and a packaging device 100 for sequentially packaging each of the multiple cell masses.

An ultrasonic cellular stimulation device includes: an ultrasonic transducer for generating a vibration wave; an upper cap having a fixing portion disposed on a lateral surface thereof; and a lower cap having a first surface and a second surface, and including: a recess indenting the first surface; an abutment portion located at a bottom of the recess; and an opening formed in the second surface and being in communication with the recess. The upper cap is partially received in the recess. The ultrasonic transducer is clamped and fixed between the fixing portion and the first surface. A glass piece is disposed on the abutment portion and fixed between the upper cap and the lower cap, such that the vibration wave is conveyed from the first surface into the abutment portion and the glass piece and a uniform and controllable sound field is generated above the glass piece.

Provided is a compound introduction apparatus including: a processing chamber; a first heater that introduces an introduction-target compound into a cell inside the processing chamber; a supply flow channel for supplying a cell suspension containing the cell and the introduction-target compound to the processing chamber; a discharge flow channel for discharging the cell suspension from the processing chamber; a second heater that prevents backflow of the cell suspension from the processing chamber into the supply flow channel; and a third heater that pressurizes the discharge flow channel.

The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alga, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described.

The present invention provides a stem cell manufacturing system comprising: a sending channel (20) through which a solution containing cells flows; an apparatus (30) which is connected to the sending channel (20) and transfers a pluripotency inducer into the cells to produce cells harboring the inducer; and an apparatus (40) which cultures the cells harboring the inducer to produce cell clusters consisting of stem cells.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.

Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention.