The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

The present disclosure provides systems and methods for host cell improvement utilizing epistatic effects. The systems and methods described herein are host cell agnostic and therefore can be implemented across taxa. Furthermore, the disclosed systems and methods can be implemented to modulate or improve any host cell parameter of interest.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

The present disclosure provides systems and methods for intracellular delivery. The systems and methods may comprise the use of a cell processing apparatus which may comprise a plurality of compression elements such as ridges. The intracellular delivery may be caused by rapid compression of cells, which may result in a reduction of a cell volume. The compression may occur while the cells pass through gaps formed by at least a subset of the ridges. The cell processing apparatus may further comprise one or more recovery spaces which are positioned between adjacent ridges. The cells may recover at least a portion of the reduced cell volume by absorbing media and/or reagents surrounding the cells while flowing through the recovery spaces. The ridges may also divert less compressible cells into a diversion channel, thereby sorting the cells based on various cell properties and/or preventing clogging within the apparatus.

A system to determine the transfection status of at least one cell in a population of cells includes a fluidic device comprising a microfluidic channel and a detection zone comprising a detection electrode module comprising two electrodes configured to detect electrical impedance between the electrodes transversely across the channel in the detection zone; corresponding to a cell passing the detection zone, a pump fluidically coupled to the fluidic device and configured to pump the population of cells in a carrier liquid along the microfluidic channel, and a processor operatively coupled to the detection electrode module and configured to detect a change in electrical impedance corresponding to a cell passing the detection zone, compare the change in electrical impedance with a reference change in electrical impedance corresponding to a cell of known transfection status, and calculate the transfection status of the cell based on the comparison.

The present invention features the use of cavity acoustic transducers (CATs) to apply mechanical stimuli on cells. CATs utilize the generated acoustic microstreaming vortices to trap cells and apply tunable shear on them. The present invention may use such a portable, automated, and high throughput device for cell transfection.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

This disclosure relates generally to containers (such as bags) having surfaces comprising one or more aptamer sequences. More particularly, the present disclosure relates to containers such as bags comprising a fluoropolymer attached to aptamer sequences having binding affinity for one or more biological agents, and to transductions methods using such containers. In one aspect, the disclosure provides a container (e.g., a bag) having an outer surface and an inner surface, the inner surface comprising a fluoropolymer; attached to the fluoropolymer, a plurality of functional groups; and attached to each of at least a portion of the functional groups, a first aptamer sequence having a binding affinity for a viral vector.

The present disclosure provides systems and methods for host cell improvement utilizing epistatic effects. The systems and methods described herein are host cell agnostic and therefore can be implemented across taxa. Furthermore, the disclosed systems and methods can be implemented to modulate or improve any host cell parameter of interest.