The invention herein describes a novel photobioreactor (PBR) system for culturing algae. The PBR system utilizes vertical transparent tubes that can be cyclically filled with an algae culture. The vertical tubes further feature pipe pigs deployed therein to disrupt formation of biofilm on the inner walls during the cyclic filling and emptying of the cultures.

A cell culture apparatus comprises at least one storage space, at least one barrier, a working space, a sterilization space, and a control space. The at least one storage space comprises a container shelf, an upright track, a rotatable holder, and a light tube. The working space comprises a filling station and an inspecting station. The filling station comprises a first delivering device, an open-close device, and a filling mechanism. The inspecting station comprises a first inspecting mechanism and a second delivering device. Also provided is a method of using the cell culture apparatus including steps of filling, inspecting, or collecting cell cultures, therefore, the cell culture apparatus performs multiple cell culture processes automatically.

A dispensing system has a sterilizable chamber. At least one robotic arm assembly and a dispensing device are within the sterilizable chamber. The dispensing device comprising a first dispensing element and a second dispensing element. The first dispensing element is connectable to the second dispensing element The at least one robotic assembly is configured to move the dispensing device. An external control device is connected to the at least one robotic arm assembly and is configured to control the at least one robotic arm assembly. The first dispensing element is configured to dispense a structural material and the second dispensing element is configured to dispense a biological material.

The present invention relates to a hybrid aseptic connection system and aseptic connection method for manufacturing an antibody pharmaceutical. In a hybrid system employing a medium storage tank made of a SS material and a bioreactor made of an SU disposable tube, to ensure a completely aseptic connection between the medium storage tank and the bioreactor, the completely aseptic connection between the medium storage tank and the bioreactor may be made by interconnection of the SU disposable tube through a welding process and the advantages of both the SS system and the SU system may be obtained at the same time. As a discharge line, an SU disposable tube and a steam trap tube are formed in a “Y” shape, the flowability in a medium transfer line may be improved and thus it is possible to prevent impurities such as a condensate from remaining in the medium transfer line.

Provided herein is a column-based continuous viral inactivation system, comprising one or both of a pH feedback controller to adjust feed flow rates and a minimum residence time (MRT) feedback controller to adjust feed flow rates. Methods of viral inactivation with the system are also provided.

Laminar air flow workstation, comprising: a working chamber (1) comprising a work table (2), an air circulation system (3) comprising first heating means (4), and configured to circulate heated air in the workstation and direct a heated and temperature controlled air flow towards the work table (2), wherein the working chamber comprises a front handling opening (6) in fluid connection with the surroundings (7), and configured such that the work table (2) can be accessed from the surroundings (7). Also disclosed are the use of the workstation for handling and/or microscopy of biological materials, such as tissue, embryos, and stem cells, as well as the use of the workstation for microbiological safety workstation in accordance with ISO/EN 12469.

Disclosed herein are cell processing systems, devices, and methods thereof. A system for cell processing may comprise a plurality of instruments each independently configured to perform one or more cell processing operations upon a cartridge, and a robot capable of moving the cartridge between each of the plurality of instruments.

An operation isolator forms an aseptic space. An incubator is connected to the operation isolator, in which cells are stored and cultured. A storage chamber stores articles used in the operation isolator. In order to carry articles from the outside into the storage chamber, a decontamination pass-box is provided. The storage chamber and the operation isolator are directly or indirectly connected to each other.

Various embodiments of the present technology provide methods and systems for soil enrichment. The systems may comprise a bioreactor system coupled to an initial treatment system for the cultivation of a live microorganism culture containing organic nutrients on an agriculturally effective scale. The systems may be automated and/or portable for practical applications onto target fields. The live microorganism culture may be delivered onto the soil of the target fields, enriching the soil with the organic nutrients that become bioavailable to crops growing in the soil. The soil enrichment system may provide a sustainable approach to agriculture that may efficiently enhance the natural processes of the native soil of any crop.

Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.