The invention relates to a biogas plant (1) of the type comprising, at least, one anaerobic digester (2) to obtain biogas from organic waste material (3), and which further comprises: at least one graphene synthesis reactor (4), and a biogas supply (5) as a source of carbon for said reactor (4), coming from the digester (2).

Nucleic acid-guided nuclease editing in mammalian cells may include passaging mammalian cells, in an automated closed cell editing instrument, into smaller aggregates when the aggregates exceed 50-300 microns in size. A library of viral particles may be delivered to the mammalian cells at a multiplicity of infection such that each mammalian cell receives one or no viral particle. The library may include viral vectors with an editing cassette including a pair of gRNA coding sequence and donor DNA. Conditions may be provided to allow a viral vector of the viral vectors to integrate into the mammalian cells. Enriching for mammalian cells may be done with an integrated viral vector. A nucleic acid-guided nuclease or nuclease fusion or a coding sequence for a nucleic acid-guided nuclease or nuclease fusion may be delivered to the enriched mammalian cells and conditions may be provided to allow editing in the mammalian cells.

A wood chip fermentation device includes: a chip fermenter 11 that is charged with wood chips serving as a heat source and that ferments the wood chips; a temperature sensor 11a that is provided inside the chip fermenter 11 and measures the temperature inside the chip fermenter; a thermal energy extracting pipe 12 that is disposed inside the chip fermenter and extracts fermentation heat from inside the chip fermenter; a pump section 13 that supplies a medium to or takes out a medium from the thermal energy extracting pipe 12; a stirring blade 14a that stirs wood chips 10a put in the chip fermenter 11; and a discharge conveyor 15 that is disposed at a lower portion of the chip fermenter 11 and discharges fermented material. The stirring blade 14a is disposed between the thermal energy extracting pipes 12 and a rotating surface thereof is in a state of being parallel to the thermal energy extracting pipes 12. A bottom surface of the chip fermenter 11 is formed in an arcuate shape such as to follow the rotating outer peripheral surface of the stirring blade 14a. A plurality of temperature sensors 11a are installed in a vertical direction inside each chip fermenter. Control of an on-off valve of the thermal energy extracting pipe 12 is performed based on temperature information from the temperature sensors 11a.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

A method of using electricity to produce methane includes maintaining a culture comprising living methanogenic microorganisms at a temperature above 50 C. in a reactor having a first chamber and a second chamber separated by a proton permeable barrier, the first chamber comprising a passage between an inlet and an outlet containing at least a porous electrically conductive cathode, the culture, and water, and the second chamber comprising at least an anode. The method also includes coupling electricity to the anode and the cathode, supplying carbon dioxide to the culture in the first chamber, and collecting methane from the culture at the outlet of the first chamber.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.

Culture systems and methods of using same. The systems include a housing defining an inner space. The inner space includes a headspace and at least a portion of a reservoir. A surface for immobilizing cells is moveable between the headspace and the reservoir. The systems can be used for coculturing methanotrophs and phototrophs for processing biogas and wastewater, particularly from anaerobic digesters.

The claimed invention is directed towards a novel combination cell electroporation/cell culturing apparatus which can be termed a cell culture dish suitable for in vitro electroporation, and towards a device suitable for in vivo electroporationboth useful in methods suitable for the generation of developmentally-activated, pluripotent, pluripotent-like, multipotent, and/or self-renewing cells which are capable of beginning to differentiate in culture into a variety of cell types and capable of further differentiation in vivo. The claimed invention is also directed towards the generation of desirable, differentiating somatic cell populations transplantable to animals or patients, genetic modification of endogenous and exogenous cells, and the treatment of patients suffering from diseases that may be ameliorated by these methods. This invention also provides methods for preventing, treating, or retarding disease, for example, immunodeficiency virus (e.g. HIV-1, HIV-2, SIV, FIV, etc.) infection.

According to present disclosure, there is disclosed an algae growth and cultivation system that provides a cost-efficient means of producing algae biomass as feedstock for algae-based products, such as, fertilizer, feed, biofuel manufacture, and desirably impacts, nutrient recovery from waste streams for valued byproducts production, recycle water, and alternative/renewable energy production. The system as discussed herein is an integrated systems approach to wastewater treatment, algal strains selection for byproducts production, and recycle of algal biomass-processing waste or additional algae harvested as feedstock for products such as fertilizer production. Embodiments of a system as discussed herein present an economically viable algae production system and process that allows algae-derived products such as fertilizer, feed, biofuels, etc. to compete with non-organic or petroleum products in the marketplace.