The process for producing organic molecules from fermentable biomass includes a step of anaerobic fermentation (5) producing volatile fatty acids (6), these precursors being transformed into final organic molecules by non-fermentation means. It also includes at least the following steps: a) extracting (9) at least one portion of the volatile fatty acids from the fermentation medium in such a way that the production of fermentation metabolites by the microorganisms (M) is not affected, and introducing a portion of the liquid phase (11) containing microorganisms from the extraction (9), b) synthesizing (13) organic molecules from the fermentation metabolites or from the volatile fatty acids extracted in step a)-c) continuing steps a) to b) until the final molecules are obtained, in terms of amount and quality. The invention also relates to an installation for implementing the process.

A method of culturing cells including: dividing a culture area into a center area and a plurality of peripheral areas, and designating the center area and the plurality of peripheral areas as measurement positions; calculating: a confluent rate at each of the measurement positions designated; and an average confluent rate which is an average of sum of the confluent rates at the measurement positions designated, the confluent rate being defined as a proportion of an area occupied by cells in a designated area; and determining a timing to perform a subculture of the cells, based on the confluent rate, wherein the determining of the timing further includes determining when the average confluent rate is smaller than a first threshold value, the confluent rate of the center area is larger than a second threshold value, and the second threshold value is larger than the first threshold value.

A pack includes at least one stack with Petri dishes which extends in a longitudinal direction, a connecting device which holds the at least one stack together, and an outer packaging which surrounds the at least one stack and which can be opened to remove the at least one stack.

An automated method includes providing a dry ingredient to be reconstituted into a liquid bioprocess solution and controlling, by a processing circuit, all automated system including at least one mixing chamber, an array of tubing for fluid flow within the system, and a plurality of valves provided within the tubing, to automatically prepare the liquid bioprocess solution from the dry ingredient. Controlling the auto system may include performing a series of sequential mixing steps, the series of sequential mixing steps causing the preparation of the liquid bioprocess solution. The method mixing include taking one or more measurements during the preparation of the liquid bioprocess solution, wherein each step is triggered by at least one of a measurement decreasing below, equaling, or exceeding a measurement threshold. Each step may also include opening or closing, by the processing circuit, at least one of the plurality of valves.

The present disclosure provides a bio-ink cartridge, a bio-ink cartridge assembly, a microsphere preparation device, a shell assembly device, a bio-block preparation instrument, a bio-ink preparation instrument and a bio-ink preparation system. The bio-ink cartridge comprises an ink cartridge shell with a cavity; a dividing wall arranged in the cavity and dividing the cavity into a first accommodating chamber and a second accommodating chamber independently; a first ink cartridge inlet and a first ink cartridge outlet which are arranged on the ink cartridge shell, communicated with the first accommodating chamber and separated from the second accommodating chamber; a second ink cartridge inlet and a second ink cartridge outlet which are arranged on the ink cartridge shell and communicated with the second accommodating chamber; and an inner flow channel located in the second accommodating chamber and communicated with the first accommodating chamber and the first ink cartridge outlet. The present disclosure is conducive to improve efficiencies of microsphere preparation, shell assembly and bio-block preparation. Further, the present disclosure is also conducive to improving activity of bio-block and intermediate products thereof.

Apparatuses, systems, and methods are provided for culturing a buoyant target tissue using a sandwich construct. Embodiments include preparing a buoyant target tissue in a sandwich construct for shipment, and preparing a sandwich construct comprising live tumor tissue for evaluation of candidate therapies for treating the tumor. The buoyant target tissue may include various cell types including cancer cells and tumors.

A buffer management system includes at least one workstation configured to support a supply of concentrated buffer solution and an inline dilution skid having a manifold and at least one workstation to support delivery of diluted (surge) buffers from the inline dilution skid. The latter workstation also serves to supply ready-made (diluted) buffers to a bioprocessing unit. The manifold is in fluid communication with the supply of concentrated buffer solution and is adapted to be placed in fluid communication with a source of water for injection (WFI). The inline dilution skid is configured to variably mix the supply of concentrated buffer solution and the source of WFI to obtain a range of diluted buffer solutions. Processes for automatically identifying the arrangement of buffer solutions in a bioprocessing application use signals from various sensors to identify the fluid connections between components of the system.

A buffer management system includes at least one workstation configured to support a supply of concentrated buffer solution and an inline dilution skid having a manifold. The manifold is in fluid communication with the supply of concentrated buffer solution and is adapted to be placed in fluid communication with a source of water for injection (WFI). The manifold includes a buffer inlet line in selective fluid communication with the supply of concentrated buffer solution, a WFI inlet line in selective fluid communication with the source of WFI, and a discharge line in fluid communication with both the buffer inlet line and the WFI inlet line. The inline dilution skid is configured to variably mix the supply of concentrated buffer solution and the source of WFI to obtain a range of diluted buffer solutions.

A holder (10) receives and stores laboratory vessels for samples, microorganisms, cell cultures or the like, with a housing (14), and at least one receptacle (20) for the laboratory vessels. The receptacle (20) extends along a loading axis (L) in the housing (14) and is configured such that the laboratory vessels can be introduced into the receptacle (20) and can be stacked on top of each other therein, wherein the receptacle (20) in the housing (14) has a loading opening (20a) to permit loading. On an underside (18) arranged remote from the loading opening (20a), the receptacle (20) has an unloading opening (20b), which can be closed by means of a closure mechanism (38) arranged in the housing (14). The closure mechanism (38) is provided with a slide (40, 42), via which the slide (40, 42) is movable to the unloading opening (20b) to permit closure and is movable from the unloading opening (20b) to an opening position to permit opening.

A pressurized appliance comprising, a tank, suitable for receiving liquid contents, a cover, suitable for sealing the tank in a closed position, said cover comprising a sampling tip, said tip comprising a cannula capable of being in contact with the contents when the cover is in the closed position, the sampling tip comprising at least one sealable orifice making it possible, when the cover is in its closed position and said orifice is a through-orifice, to ensure a pressure equilibrium between the inside of the cannula and the inside of the tank, and when the cover is in its closed position and said orifice is sealed, to sample the contents via the cannula.