Provided are a cell dispensing and storing apparatus and a method of the same. The method includes: moving a freezing container with a test tube in it and a temporary storage bottle containing sample cells into a dispensing area. Connect the temporary storage bottle with an injecting assembly. A dispensing clamp moves the test tube from the freezing container to a tube rack on a rotating platform. A rotating clamp removes a tube cover from the test tube. An injecting nozzle extracts the sample cells from the temporary storage bottle and dispenses the sample cells into the test tube. The rotating clamp puts the tube cover back on the test tube. The dispensing clamp moves the test tube back into the freezing container. A freezing clamp moves the freezing container into a freezer.

An automated apparatus for preparing a liquid bioprocess solution includes at least one mixing chamber having a lower port and an upper port for fluid to enter the at least one mixing chamber, an array of tubing for fluid flow within the system, a plurality of valves provided within the tubing, and a mixing controller configured to cause the automated apparatus to perform a series of sequential mixing steps causing the preparation of the liquid bioprocess solution from a dry ingredient. The series of sequential mixing steps include opening a first valve associated with the lower port to provide fluid to the at least one mixing chamber through the lower port, and after a predetermined amount of elapsed time, closing the first valve and opening a second valve associated with the upper port to provide fluid to the at least one mixing chamber through the upper port.

System for performing a media preparation process controlled by a computer (7) further comprising a Media Preparation Module (12), a media bag (2) for storing the media in an unprepared state (13), a Dispensing Module (9, 9a) for storing the media in a finally prepared state (17), and a water providing system which connects the Media Preparation Module (12) with the media bag (2) and the Dispensing Module (9, 9a) and provides them with temperature adjustable water using a water sterilization filter set (4) wherein, the system (6) additionally comprises an attachable machine readable data storage (3) which contains information about the current state and/or operating parameters of the system parts, which are read, processed and used by the computer (7) to operate the system (6) according to required conditions.

A buffer management system includes at least one workstation configured to support a supply of concentrated buffer solution and an inline dilution skid having a manifold and at least one workstation to support delivery of diluted (surge) buffers from the inline dilution skid. The latter workstation also serves to supply ready-made (diluted) buffers to a bioprocessing unit. The manifold is in fluid communication with the supply of concentrated buffer solution and is adapted to be placed in fluid communication with a source of water for injection (WFI). The inline dilution skid is configured to variably mix the supply of concentrated buffer solution and the source of WFI to obtain a range of diluted buffer solutions. Processes for automatically identifying the arrangement of buffer solutions in a bioprocessing application use signals from various sensors to identify the fluid connections between components of the system.

The invention relates to a tubing device (40) for sterile media comprising a container (54) for storing sterile media, a discharge port (52) for discharging liquid sterile media, an inlet port (41) for introducing fluid into the tubing device and a multiple-way-connector (50), wherein the multiple-way-connector (50) is connected to the container (54), the discharge port (52) and the inlet port (41), and an apparatus (80) for preparing, mixing and discharging a sterile medium for use with a tubing device (40), comprising a water inlet port (41) for introducing water, a water outlet port (87) configured to be connected to the inlet port (41) of the tubing device (40), a fluid pump (82) for conveying the fluid, the fluid pump (82) is connected to the fluid inlet port (81) and the water outlet port (87) and a container support (88) for supporting the container (54) of the tubing device (40).

The present disclosure is directed to the organic growth and phasing design for a system for the industrial growth of biologics. A system may include a number of subsystems, such as a buffer distribution subsystem, a media preparation subsystem, a bioreactor subsystem, a harvest subsystem, and/or a purification subsystem. The subsystems may be highly interconnected for flexible process flow design. Additionally, the subsystems may be constructed with a total output capacity with a total number of equipment stations and operated at a lower output capacity with fewer than the total equipment stations to maintain headroom for growth in phases.

A method for providing a large amount of cell culture medium (10), in particular for cultivating animal cells, for a large reactor system (12) by using single-use components, comprises the following sequence of steps: a) preparing a concentrated media stock solution (32), b) filling the concentrated media stock solution (32) into single-use containers (40), c) transporting the single-use containers (40) filled with media stock solution (32) to the large reactor system (12), d) aseptically connecting the single-use containers (40) to the large reactor system (12) by means of a single-use hose system (26), e) processing the media stock solution (32) by supplying ultrapure water (24) and/or additives (44), and f) supplying the processed media stock solution (32) into the large reactor system (12).

A dissolution system includes a dissolution line and a bypass line. The dissolution line fluidly couples a biocontainer, a pump, a cartridge, an upstream filter, and a downstream filter in a circulation loop. The bypass line is in fluid communication with the dissolution line at an upstream junction and a downstream junction such that the bypass line is in parallel relationship with the cartridge. A flow control system is configured to control the flow of fluid through the cartridge such that the flow of fluid through at least one of the dissolution line and the bypass line is selectively controlled based upon a pressure in the dissolution line downstream of the cartridge between the cartridge and the biocontainer.

The present invention relates to a method of analysis comprising the preparation and the analysis of a sample in a flexible container without direct handling of the sample and without reopening the flexible container at the end of the preparation of the sample, and also to a container and a kit that enable the implementation of this method.

This invention concerns methods of packaging and shipping stem cells. Also disclosed are related package products.