An solution transfer device comprises a pump 60 and a substrate 70. The pump 60 comprises a tube 1 for transferring a solution; tube rotors 21A, 21B, 21C, which contact the tube 1; and a driver 10 for transferring a solution within the tube 1 by rotating the tube rotors 21A, 21B, 21C without contacting the tube rotors 21A, 21B, 21C. The substrate 70 is provided with a solution-transferring flow path that is connected to the tube 1 of the pump 60.

An solution transfer device comprises a pump 60 and a substrate 70. The pump 60 comprises a tube 1 for transferring a solution; tube rotors 21A, 21B, 21C, which contact the tube 1; and a driver 10 for transferring a solution within the tube 1 by rotating the tube rotors 21A, 21B, 21C without contacting the tube rotors 21A, 21B, 21C. The substrate 70 is provided with a solution-transferring flow path that is connected to the tube 1 of the pump 60.

Metastable state spore incubation mixing systems are described. An example system includes a spores container to store spores, a nutrient container to store nutrients, a water supply line, a syringe tank, a syringe pump, an adjustable valve, a heater, and a controller. In a drawing phase of the system, a controller can cause the syringe pump and the adjustable valve to draw into the syringe tank a volume of spores, nutrients, and water to form a mixture. The controller causes the heater to heat the mixture for a period of time. In a dispensing phase of the system, the controller can cause the syringe pump to expel the mixture through the adjustable valve and into a water distribution system. The controller can direct the system through a number of other phases of operation.

Metastable state spore incubation mixing systems are described. An example system includes a spores container to store spores, a nutrient container to store nutrients, a water supply line, a syringe tank, a syringe pump, an adjustable valve, a heater, and a controller. In a drawing phase of the system, a controller can cause the syringe pump and the adjustable valve to draw into the syringe tank a volume of spores, nutrients, and water to form a mixture. The controller causes the heater to heat the mixture for a period of time. In a dispensing phase of the system, the controller can cause the syringe pump to expel the mixture through the adjustable valve and into a water distribution system. The controller can direct the system through a number of other phases of operation.

The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using an Escherichia coli genetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on an Escherichia coli W3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

Prokaryotic cells having a metal-phenolic network coating are disclosed, as are compositions including such cells, methods for their use, and methods for producing such cells.

The present application provides a group of human immortalized B lymphocyte cell lines and use thereof, and specifically provides a combination of four closely related immortalized lymphocyte cell lines. The combination can be used as a reference substances for measuring the performance of a detection platform. When the four closely related immortalized lymphocyte cell lines are used as reference substances for epigenome, transcriptome, proteome, and metabolome, an intrinsic magnitude difference gradient can be formed to evaluate the sensitivity of histological detection.

Provided herein is a method for producing a lyophilized composition of viable cells of Rhizobium bacteria having high viable cell viability, and a lyophilized composition of viable cells of Rhizobium bacteria having desirable long-storage survivability, among others. The lyophilized composition of viable cells of Rhizobium bacteria having high viable cell viability and desirable long-storage survivability can be obtained when a composition containing viable cells of Rhizobium bacteria and more than 10 mass % moisture is lyophilized to bring the moisture content to 10 mass %, and the drying is ended before the moisture content becomes less than 5 mass % so that the composition after the lyophilization contains the viable bacteria and 5 to 10 mass % of moisture.

The present disclosure provides a simple and secure apparatus and a simple and secure method for selectively detecting a tomato pathogenic fungus. The tomato pathogenic fungus detecting apparatus according to the present disclosure is characterized by including an artificial cell wall, a test sample solution inlet provided above the artificial cell wall, and a culture solution storage part provided under the artificial cell wall, wherein a test sample solution contains a 50 mM to 70 mM buffer solution of a citrate salt in the test sample solution inlet, and the test sample solution has a pH of 5 to 5.5.

Arginine deiminase (ADI)-containing genetically engineered Corynebacterium glutamicum (C. glutamicum), a fusion protein cipA-arc, use thereof, and a method for preparing [.sup.14/15N]-L-citrulline through enzymatic catalysis are provided. The ADI-containing genetically engineered Corynebacterium glutamicum (C. glutamicum) has a deposit number of CGMCC No. 19404, which expresses a fusion protein cipA-arc. Both the genetically engineered strain and the fusion protein cipA-arc can be used to convert [.sup.14/15N]-L-arginine into [.sup.14/15N]-L-citrulline.