A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41 C. to 44 C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41 C. to 44 C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41 C. to 44 C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

The present invention relates to a method and a device for fermentation based on a microbial asexual reproduction. The method comprises the steps of adding plural silica sands, a medium and a microorganism into a tank; and stirring for separating a cluster of the microorganism by a shear force generated from the plural silica sands and returning the microorganism to a logarithmic growth phase without undergoing a spore phase to increase a fermentation rate of the microorganism. The device comprises a tank, a speed control motor disposed outside the tank, a stirring component connected to the speed control motor, a refluxing mechanism for high pressure air and water connected to the tank, a heating unit disposed in the tank and a pumping motor connected to the tank.

Described in this application is a method for producing Plasmodium sporozoites in a cell-free, mosquito-free in vitro culture-system. Compositions and methods resulting from applying the methods of the present invention are described.

Described in this application is a method for producing Plasmodium sporozoites in a cell-free, mosquito-free in vitro culture-system. Compositions and methods resulting from applying the methods of the present invention are described.

This invention discloses a mutated strain of wild type Bacillus mucilaginosus HSCUP-76-8 and a high-density fermentation method thereof. The mutated strain HSCUP-76-8 was assigned an Accession No. CGMCC No. 8481. A two-stage and regulated high-density fermentation method has been established for the production of HSCUP-76-8. In the first stage, parameters are controlled to reduce the viscosity of the fermentation broth and promote the growth of bacteria, thereby allowing the bacteria to reach an amount in the range of 2.010.sup.9 cfu/mL-2.310.sup.9 cfu/ml. In the second stage, nutritional factors and fermentation conditions are controlled to promote sporulation, thereby producing endospores in the range of 1.510.sup.9 cfu/mL-2.010.sup.9 cfu/ml. The fermentation cycle of the two-stage high-density fermentation is 32-48 hours.

This invention discloses a mutated strain of wild type Bacillus mucilaginosus HSCUP-76-8 and a high-density fermentation method thereof. The mutated strain HSCUP-76-8 was assigned an Accession No. CGMCC No. 8481. A two-stage and regulated high-density fermentation method has been established for the production of HSCUP-76-8. In the first stage, parameters are controlled to reduce the viscosity of the fermentation broth and promote the growth of bacteria, thereby allowing the bacteria to reach an amount in the range of 2.010.sup.9 cfu/mL-2.310.sup.9 cfu/ml. In the second stage, nutritional factors and fermentation conditions are controlled to promote sporulation, thereby producing endospores in the range of 1.510.sup.9 cfu/mL-2.010.sup.9 cfu/ml. The fermentation cycle of the two-stage high-density fermentation is 32-48 hours.

The invention relates to methods for inducing sporulation in Bacillus coagulans strains wherein excessive sporulation is induced by presence of certain nutrients and minerals up to a level of 109 spores/ml. The spores are tadpole-like endospores and the method discloses specific parameters of growth medium and incubation conditions to be used to induce sporulation in Bacillus coagulans strains.

The invention relates to methods for inducing sporulation in Bacillus coagulans strains wherein excessive sporulation is induced by presence of certain nutrients and minerals up to a level of 109 spores/ml. The spores are tadpole-like endospores and the method discloses specific parameters of growth medium and incubation conditions to be used to induce sporulation in Bacillus coagulans strains.