The present invention provides a method for determining whether or not a test sample contains a phytopathogenic oomycete selectively from two kinds of oomycetes of a phytopathogenic oomycete and a non-phytopathogenic oomycete. The method according to the present invention comprises: (a) putting the test sample on a front surface of a cellulose film; wherein the cellulose film has a thickness of not less than 0.5 micrometers and not more than 3.7 micrometers; (b) leaving the test sample at rest for more than 8 hours and not more than 12 hours after the step (a); (c) observing a back surface of the film after the step (b); and (d) determining that the test sample contains the phytopathogenic oomycete, if an oomycete is found on the back surface of the film in the step (c).

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifications of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

The present disclosure provides an entomopathogenic fungi conidia powder, which comprises a powder and conidia attached to the surface of the powder. On the other hand, the present disclosure further provides a method for producing the entomopathogenic fungi conidia powder. Based on the disclosure of the present disclosure, the production method uses a powder to adhere to the entomopathogenic fungi conidia, and allowing them to be stored at room temperature for at least 7 months with a substantially same amount of conidia population. In addition, the production method using solid fermentation reduces production costs.

The present disclosure provides an entomopathogenic fungi conidia powder, which comprises a powder and conidia attached to the surface of the powder. On the other hand, the present disclosure further provides a method for producing the entomopathogenic fungi conidia powder. Based on the disclosure of the present disclosure, the production method uses a powder to adhere to the entomopathogenic fungi conidia, and allowing them to be stored at room temperature for at least 7 months with a substantially same amount of conidia population. In addition, the production method using solid fermentation reduces production costs.

The present invention relates to methods of aseptically producing filamentous fungi in a bioreactor by pseudo-solid state fermentation. The present invention further relates to methods of continuously, aseptically producing filamentous fungi in a bioreactor by pseudo-solid state fermentation.

The present invention relates to methods of aseptically producing filamentous fungi in a bioreactor by pseudo-solid state fermentation. The present invention further relates to methods of continuously, aseptically producing filamentous fungi in a bioreactor by pseudo-solid state fermentation.

A method of pH regulating biomass residues includes the steps of: providing biomass residue comprising solid and liquid parts; exposing the biomass to one or more separation steps, wherein said biomass is separated into one or more solid fractions having a reduced content of water, and one or more fluid fractions; and adding a pH regulator to the biomass residue, and/or the one or more solid fractions, and/or the one or more fluid fractions, wherein the pH regulator comprises a juice derived from agricultural biomass and/or fish manure.

A method of pH regulating biomass residues includes the steps of: providing biomass residue comprising solid and liquid parts; exposing the biomass to one or more separation steps, wherein said biomass is separated into one or more solid fractions having a reduced content of water, and one or more fluid fractions; and adding a pH regulator to the biomass residue, and/or the one or more solid fractions, and/or the one or more fluid fractions, wherein the pH regulator comprises a juice derived from agricultural biomass and/or fish manure.

Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.