The present invention refers to a process designed to increase the production of plant growth-promoting microorganisms of the Bacillus genus, using a culture medium poor in nutrients and with specific environmental conditions, allowing to obtain a greater amount of biomass and/or spores, which can be used to prepare solid or liquid compositions to be applied to plants, aiming to promote their growth and/or counteract the effect of phytopathogenic agents.

Fusion proteins, recombinant Bacillus cereus family members that express fusion proteins, and exosporium fragments derived from spores of the recombinant Bacillus cereus family members are provided. Compositions comprising the spores or exosporium fragments are also provided. Methods involving the use of spores of recombinant Bacillus cereus family members and exosporium fragments derived from spores of a recombinant Bacillus cereus family member in the fields of animal health and aquaculture are provided. In particular, methods are for provided for using such spores or exosporium fragments for protecting an animal or an aquatic organism from a pathogen. Methods are also provided for using exosporium fragments for producing an immunogenic response in an aquatic animal. Products for use in protecting animals from pathogens are also provided, including adhesive patches, wound dressings, insert trays for livestock footbaths, hoof bandages, feed, feed additives, and insect foggers.

The biological functionality of living microbial spores is modified using phenotypic engineering to endow the resulting modified spores with novel functionality that extends the usefulness of the spores for a variety of practical applications including, for example, sterility testing, the release of active compounds, and cell-based biosensing systems. An embodiment entails engineering Bacillus spores to acquire synthetic new functions that enable the modified spores to sense and rapidly transduce specific germination signals in their surroundings. The newly acquired functions allow the spores to perform, for example, as self-reporters of cellular viability, self-indicating components of cell-based biosensors, and in other analytical systems. Also disclosed are methods for testing adequate sterility of a system by using engineered spores.

The biological functionality of living microbial spores is modified using phenotypic engineering to endow the resulting modified spores with novel functionality that extends the usefulness of the spores for a variety of practical applications including, for example, sterility testing, the release of active compounds, and cell-based biosensing systems. An embodiment entails engineering Bacillus spores to acquire synthetic new functions that enable the modified spores to sense and rapidly transduce specific germination signals in their surroundings. The newly acquired functions allow the spores to perform, for example, as self-reporters of cellular viability, self-indicating components of cell-based biosensors, and in other analytical systems. Also disclosed are methods for testing adequate sterility of a system by using engineered spores.

The present invention relates to a method of producing fungal spores with prolonged storage stability or of prolonging the storage stability of fungal spores comprising subjecting fungal spores to a drying procedure to result in a residual moisture content of 12% or less and a composition comprising fungal spores obtained by said method.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

A probiotic composition is provided comprising Bacillus amyloliquefaciens strain H57 bacteria that confers health and/or nutritional benefits, including methods of producing and using such a composition. Also provided is a method of treating a 5 probiotic-responsive disease, disorder and condition in an animal and a method of modulating the gastrointestinal flora of an animal, both including administering to said animal a probiotic composition comprising Bacillus amyloliquefaciens strain H57 bacteria.

A probiotic composition is provided comprising Bacillus amyloliquefaciens strain H57 bacteria that confers health and/or nutritional benefits, including methods of producing and using such a composition. Also provided is a method of treating a 5 probiotic-responsive disease, disorder and condition in an animal and a method of modulating the gastrointestinal flora of an animal, both including administering to said animal a probiotic composition comprising Bacillus amyloliquefaciens strain H57 bacteria.

A concentrated bioenzymatic cleaning product is provided which maintains a homogenous composition under static conditions and does not require shaking or mixing prior to dilution. The concentrated bioenzymatic cleaning product includes bacillus spores, a suspending and/or rheology modifying agent, an odor control agent, a pH adjuster, and water. The types and amounts of ingredients are selected to maintain the bacillus spores in suspension under static conditions, enabling the concentrated bioenzymatic cleaning product to be diluted using a proportioner system.

A concentrated bioenzymatic cleaning product is provided which maintains a homogenous composition under static conditions and does not require shaking or mixing prior to dilution. The concentrated bioenzymatic cleaning product includes bacillus spores, a suspending and/or rheology modifying agent, an odor control agent, a pH adjuster, and water. The types and amounts of ingredients are selected to maintain the bacillus spores in suspension under static conditions, enabling the concentrated bioenzymatic cleaning product to be diluted using a proportioner system.