The present invention provides a preparation method of Beauveria bassiana (Bals.) Vuill microsclerotium and a formulation thereof, and an application of the formulation thereof, belonging to the technical field of microsclerotium formulation preparation; the Beauveria bassiana microsclerotium formulation is prepared by the following steps of: 1) mixing conidia of Beauveria bassiana with Tween® 20 aqueous solution to obtain spore suspension; 2) inoculating the prepared spore suspension into a liquid induction medium for induction culture to obtain microsclerotium; 3) mixing the prepared microsclerotium with a padding for drying to obtain dried microsclerotium; 4) mixing the prepared dried microsclerotium with an auxiliary to obtain a microsclerotium formulation. The Beauveria bassiana microsclerotium formulation produced by the method has low production cost, strong product tolerance and good insecticidal activity, moreover, after rehydrated, the formulation may rapidly germinate hyphae and produce spores to infect pests.

A liquid sporulation method is described. A first liquid culture is prepared by adding bacterial cells to a sporulation broth, wherein an optical density (OD.sub.600) of the first liquid culture is in a range of 0.001 to 0.01. The first liquid culture is incubated and an optical density (OD.sub.600) thereof is increased to be in a range of 0.2 to 2.0. Second liquid culture is prepared by adding the incubated first liquid culture to a predetermined amount of additional sporulation broth, wherein an optical density (OD.sub.600) of the second liquid culture is in the range of 0.001 to 0.1, and a ratio of a volume of the second liquid culture to a volume of the first liquid culture is in a range of 10:1 to 150:1. The second liquid culture is incubated so an optical density (OD.sub.600) thereof is increased to be in a range of 1.0 to 4.0.

A liquid sporulation method is described. A first liquid culture is prepared by adding bacterial cells to a sporulation broth, wherein an optical density (OD.sub.600) of the first liquid culture is in a range of 0.001 to 0.01. The first liquid culture is incubated and an optical density (OD.sub.600) thereof is increased to be in a range of 0.2 to 2.0. Second liquid culture is prepared by adding the incubated first liquid culture to a predetermined amount of additional sporulation broth, wherein an optical density (OD.sub.600) of the second liquid culture is in the range of 0.001 to 0.1, and a ratio of a volume of the second liquid culture to a volume of the first liquid culture is in a range of 10:1 to 150:1. The second liquid culture is incubated so an optical density (OD.sub.600) thereof is increased to be in a range of 1.0 to 4.0.

Metastable state spore incubation mixing systems are described. An example system includes a spore container to store spores, a nutrient container, an arrangement of valves and tubes, a reciprocating pump, a mixing tube, and a holding tank. In a drawing phase of the system, a controller can control the reciprocating pump to draw a ratioed volume of the spores, the nutrients, and water through the valves and tubes. During an expelling phase of the system, the controller can control flow control valves to direct the spores, nutrients, and water through the mixing tube and into the holding tank. The controller can also direct a heater to heat the mixture in the holding tank to a predetermined temperature. Once the mixture reaches the temperature, the controller can also direct the system through a number of other phases of operation, including cooling and purging phases.

Metastable state spore incubation mixing systems are described. An example system includes a spore container to store spores, a nutrient container, an arrangement of valves and tubes, a reciprocating pump, a mixing tube, and a holding tank. In a drawing phase of the system, a controller can control the reciprocating pump to draw a ratioed volume of the spores, the nutrients, and water through the valves and tubes. During an expelling phase of the system, the controller can control flow control valves to direct the spores, nutrients, and water through the mixing tube and into the holding tank. The controller can also direct a heater to heat the mixture in the holding tank to a predetermined temperature. Once the mixture reaches the temperature, the controller can also direct the system through a number of other phases of operation, including cooling and purging phases.

The invention is directed to compositions, tools and methods for the manufacture of construction materials, masonry, solid structures and compositions to facilitate dust control. More particularly, the invention is directed to the manufacture of bricks, masonry and other solid structures using small amount of aggregate material that is pre-loaded with spores and/or vegetative bacterial cells.

The invention is directed to compositions, tools and methods for the manufacture of construction materials, masonry, solid structures and compositions to facilitate dust control. More particularly, the invention is directed to the manufacture of bricks, masonry and other solid structures using small amount of aggregate material that is pre-loaded with spores and/or vegetative bacterial cells.

The technology described herein is directed to compositions and methods for determining provenance of an item, a non-limiting example being a food item. In one aspect described herein is an engineered microorganism comprising at least one genetic barcode element, essential gene mutations, and/or germination gene mutations. In another aspect described herein is a method of determining the provenance of an item comprising contacting the item with an engineered microorganism and later detecting the genetic barcode element to determine the provenance of the item. In another aspect, described herein is a method of determining the path of an item or individual across a surface.

The technology described herein is directed to compositions and methods for determining provenance of an item, a non-limiting example being a food item. In one aspect described herein is an engineered microorganism comprising at least one genetic barcode element, essential gene mutations, and/or germination gene mutations. In another aspect described herein is a method of determining the provenance of an item comprising contacting the item with an engineered microorganism and later detecting the genetic barcode element to determine the provenance of the item. In another aspect, described herein is a method of determining the path of an item or individual across a surface.

Devices for collecting bioaerosols are provided, including a cassette comprising a mesh made of electrostatically charged fibers held taut between a pair of mating members for supporting the collection medium in a extended position to expose a capture surface for capturing bioaerosols. The cassette is replaceably inserted in a wind vane apparatus which directs airflow to the capture surface of the cassette for capturing bioaerosols.