Devices for collecting bioaerosols are provided, including a cassette comprising a mesh made of electrostatically charged fibers held taut between a pair of mating members for supporting the collection medium in a extended position to expose a capture surface for capturing bioaerosols. The cassette is replaceably inserted in a wind vane apparatus which directs airflow to the capture surface of the cassette for capturing bioaerosols.

Herein a Bacillus exosporium antigen delivery (BEAD) system that provides a means to introduce recombinant proteins or small molecules into the exosporium of members of the B. cereus family of bacteria, i.e. B. anthracis, B. cereus, and B. thuringiensis, is disclosed. The system results in the surface display of recombinant proteins or small molecules such that they can stimulate an immune response. In addition, methods of making and using the system are described.

Herein a Bacillus exosporium antigen delivery (BEAD) system that provides a means to introduce recombinant proteins or small molecules into the exosporium of members of the B. cereus family of bacteria, i.e. B. anthracis, B. cereus, and B. thuringiensis, is disclosed. The system results in the surface display of recombinant proteins or small molecules such that they can stimulate an immune response. In addition, methods of making and using the system are described.

A spore-forming cell comprising a first nucleotide sequence encoding a riboswitch and a transcriptional activator, wherein the riboswitch modulates translation of the transcriptional activator in response to a First inducer, and a second nucleotide sequence encoding a target gene that regulates spore formation, wherein the target gene is expressed in response to a second inducer and translation of the transcriptional activator. Also provided is a method of culturing a spore-forming cell and a method of creating a spore, as well as uses of spore-forming cells and spores.

A spore-forming cell comprising a first nucleotide sequence encoding a riboswitch and a transcriptional activator, wherein the riboswitch modulates translation of the transcriptional activator in response to a First inducer, and a second nucleotide sequence encoding a target gene that regulates spore formation, wherein the target gene is expressed in response to a second inducer and translation of the transcriptional activator. Also provided is a method of culturing a spore-forming cell and a method of creating a spore, as well as uses of spore-forming cells and spores.

This invention relates to novel Penicillium strains, the production of novel Penicillium strains, and the use of novel Penicillium strains in various end applications.

This invention relates to novel Penicillium strains, the production of novel Penicillium strains, and the use of novel Penicillium strains in various end applications.

The subject invention provides methods of producing advantageous microbes and/or by-products using a modified form of solid-state fermentation, or matrix fermentation. In particular, the methods utilize foodstuff mixed with liquid nutrient medium to produce a three-dimensional scaffold substrate having ample surface area on which the microbes can grow. The methods can be used to cultivate yeasts, fungi and bacteria at high concentrations without susceptibility to total contamination. The subject invention can be used in remote locations and can be transported with ease.

This invention discloses a mutated strain of wild type Bacillus mucilaginosus HSCUP-76-8 and a high-density fermentation method thereof. The mutated strain HSCUP-76-8 was assigned an Accession No. CGMCC No. 8481. A two-stage and regulated high-density fermentation method has been established for the production of HSCUP-76-8. In the first stage, parameters are controlled to reduce the viscosity of the fermentation broth and promote the growth of bacteria, thereby allowing the bacteria to reach an amount in the range of 2.0×10.sup.9 cfu/mL-2.3×10.sup.9 cfu/ml. In the second stage, nutritional factors and fermentation conditions are controlled to promote sporulation, thereby producing endospores in the range of 1.5×10.sup.9 cfu/mL-2.0×10.sup.9 cfu/ml. The fermentation cycle of the two-stage high-density fermentation is 32-48 hours.

This invention discloses a mutated strain of wild type Bacillus mucilaginosus HSCUP-76-8 and a high-density fermentation method thereof. The mutated strain HSCUP-76-8 was assigned an Accession No. CGMCC No. 8481. A two-stage and regulated high-density fermentation method has been established for the production of HSCUP-76-8. In the first stage, parameters are controlled to reduce the viscosity of the fermentation broth and promote the growth of bacteria, thereby allowing the bacteria to reach an amount in the range of 2.0×10.sup.9 cfu/mL-2.3×10.sup.9 cfu/ml. In the second stage, nutritional factors and fermentation conditions are controlled to promote sporulation, thereby producing endospores in the range of 1.5×10.sup.9 cfu/mL-2.0×10.sup.9 cfu/ml. The fermentation cycle of the two-stage high-density fermentation is 32-48 hours.