Disclosed are a method, a composition and a device for the introduction of exogenous nucleic acids into eukaryotic cells by non-viral vectors (non-viral transfection). The method according to the invention is based on application of a high-frequency oscillatory motion to a solution containing nucleic acids and cationic polymers or lipids to obtain particles (complexes) with high transfection efficiency.

The present invention relates to a device (1) for treating cells for stimulating cell growth, comprising a unit (2) for generating and emitting electric pulses and an electric field resulting therefrom, and a treatment space (3) that can be penetrated by the emitted electric pulses, wherein the device (1) further comprises a unit (4, 9) which can be moved into and out of said treatment space (3) such that cell material provided within a compartment (4a) of said unit (4), or provided in a container (5) on or in said unit (4, 9), can be moved into and out of said treatment space (3), and wherein said treatment space (3) is provided between two electrodes (2a, 2b) or plates of a capacitor parallel to each other.

The present invention relates to a device (1) for treating cells for stimulating cell growth, comprising a unit (2) for generating and emitting electric pulses and an electric field resulting therefrom, and a treatment space (3) that can be penetrated by the emitted electric pulses, wherein the device (1) further comprises a unit (4, 9) which can be moved into and out of said treatment space (3) such that cell material provided within a compartment (4a) of said unit (4), or provided in a container (5) on or in said unit (4, 9), can be moved into and out of said treatment space (3), and wherein said treatment space (3) is provided between two electrodes (2a, 2b) or plates of a capacitor parallel to each other.

The present invention is related to a device (1), comprising a unit (2) for generating and emitting electric pulses, two or more electrodes or plates (2a, 2b) of a capacitor that are arranged in one electric circuit with said unit (2), and a treatment space (3) arranged between said electrodes or plates (2a, 2b) so that the treatment space (3) can be penetrated by the emitted electric pulses and an electric field resulting therefrom, characterized in that at least one adjustable resistor (9) is arranged in said electric circuit.

The present invention is related to a device (1), comprising a unit (2) for generating and emitting electric pulses, two or more electrodes or plates (2a, 2b) of a capacitor that are arranged in one electric circuit with said unit (2), and a treatment space (3) arranged between said electrodes or plates (2a, 2b) so that the treatment space (3) can be penetrated by the emitted electric pulses and an electric field resulting therefrom, characterized in that at least one adjustable resistor (9) is arranged in said electric circuit.

The present invention relates to a device (1) for treating cells for stimulating cell growth, comprising a unit (2) for generating and emitting electric pulses, and a treatment unit (3) having an inlet (3a) and an outlet (3a), a treatment space (4) being formed inside the treatment unit (3), the treatment space (4) being penetrated by the emitted electric pulses and an electric field resulting therefrom, a cell material entering into the treatment space (4) through the inlet (3a) being moved without contact through the treatment space (4) and the electric field penetrating the treatment space (4) to the outlet (3b).

The present invention relates to a device (1) for treating cells for stimulating cell growth, comprising a unit (2) for generating and emitting electric pulses, and a treatment unit (3) having an inlet (3a) and an outlet (3a), a treatment space (4) being formed inside the treatment unit (3), the treatment space (4) being penetrated by the emitted electric pulses and an electric field resulting therefrom, a cell material entering into the treatment space (4) through the inlet (3a) being moved without contact through the treatment space (4) and the electric field penetrating the treatment space (4) to the outlet (3b).

The present invention is related to a treatment unit (3) having an inlet (3a) and an outlet (3b), and a treatment space (4) being formed inside the treatment unit (3) such that it can be penetrated by electric pulses, said treatment space (4) being fluidly connected with the inlet (3a) and the outlet (3b), wherein at least a portion of an inner side face of the treatment space (4) is curved. The present invention is furthermore related to a device comprising such a treatment unit and to a method to be performed with said device, and wherein the inlet (3a) is formed in a top face of the treatment unit (3), and the outlet (3b) is formed in a bottom face of the treatment unit (3).

The present invention is related to a treatment unit (3) having an inlet (3a) and an outlet (3b), and a treatment space (4) being formed inside the treatment unit (3) such that it can be penetrated by electric pulses, said treatment space (4) being fluidly connected with the inlet (3a) and the outlet (3b), wherein at least a portion of an inner side face of the treatment space (4) is curved. The present invention is furthermore related to a device comprising such a treatment unit and to a method to be performed with said device, and wherein the inlet (3a) is formed in a top face of the treatment unit (3), and the outlet (3b) is formed in a bottom face of the treatment unit (3).

The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.