Patent classifications
C12N13/00
Method for extracting nucleic acids from biological sample
The present invention relates to a method for extracting nucleic acids from a biological sample, and the extraction method presents a novel method for effectively extracting nucleic acids. When nucleic acids are extracted from biological samples in the related art, various impurities present in the biological samples are not properly removed, such that the purification rate is low, but the present invention provides a method for extracting nucleic acids from a biological sample of which the bacteria, virus and nucleic acid recovery rates are enhanced, by adding a surfactant and a sodium sulfate (Na.sub.2SO.sub.4) solution in a biological sample disruption step and a purification step, thereby enabling pathogens to be detected more sensitively and accurately.
Method for extracting nucleic acids from biological sample
The present invention relates to a method for extracting nucleic acids from a biological sample, and the extraction method presents a novel method for effectively extracting nucleic acids. When nucleic acids are extracted from biological samples in the related art, various impurities present in the biological samples are not properly removed, such that the purification rate is low, but the present invention provides a method for extracting nucleic acids from a biological sample of which the bacteria, virus and nucleic acid recovery rates are enhanced, by adding a surfactant and a sodium sulfate (Na.sub.2SO.sub.4) solution in a biological sample disruption step and a purification step, thereby enabling pathogens to be detected more sensitively and accurately.
METHOD FOR SCREENING THERAPEUTIC TARGET OF ACUTE GASTROINTESTINAL SYNDROME AND USE OF TIGAR TARGET IN PREPARATION OF MEDICINE FOR TREATING RADIATION-INDUCED GASTROINTESTINAL SYNDROME
The invention discloses a method for screening a therapeutic target of acute radiation-induced gastrointestinal syndrome and use of TIGAR target in the preparation of a medicine for treating radiation-induced gastrointestinal syndrome. The CreERT-loxP transgenic mouse model is used, in which quiescent intestinal crypt stem cells are effectively promoted to proliferate after exposure to high-dose ionizing radiation, to screen a therapeutic target that still has a therapeutic effect for radiation-induced gastrointestinal syndrome 18-24 h after ionizing radiation. Gene splicing occurs in particular cells in the CreERT-loxP transgenic mice only after the injection of tamoxifen, thereby regulating gene expression. The actual situation of initial exposure and then treatment after a nuclear accident is well simulated, so the invention is of great practical significance. The screened therapeutic target is developed into a medicine for treatment after nuclear accidents, to save precious time for the treatment after nuclear accidents.
INTRACELLULAR SUBSTANCE INTRODUCTION AND EXTRACTION TECHNIQUE VIA COMPOSITE NANOTUBES
The present invention delivers a substance such as a reagent into a cell. A tubular body coated with an electroconductive polymer for introducing a substance into a cell and/or recovering a substance from a cell. The use of a tubular body coated with an electroconductive polymer to introduce a substance into a cell and/or recover a substance from a cell. A device for introducing a substance into a cell and/or recovering a substance from a cell, the device being equipped with a substrate and a tubular body coated with an electroconductive polymer, and the tubular body being positioned on the substrate. A system for introducing a substance into a cell and/or recovering a substance from a cell, the system being equipped with the abovementioned device, a voltage supply unit for supplying a voltage, and, as needed, electrodes.
INTRACELLULAR SUBSTANCE INTRODUCTION AND EXTRACTION TECHNIQUE VIA COMPOSITE NANOTUBES
The present invention delivers a substance such as a reagent into a cell. A tubular body coated with an electroconductive polymer for introducing a substance into a cell and/or recovering a substance from a cell. The use of a tubular body coated with an electroconductive polymer to introduce a substance into a cell and/or recover a substance from a cell. A device for introducing a substance into a cell and/or recovering a substance from a cell, the device being equipped with a substrate and a tubular body coated with an electroconductive polymer, and the tubular body being positioned on the substrate. A system for introducing a substance into a cell and/or recovering a substance from a cell, the system being equipped with the abovementioned device, a voltage supply unit for supplying a voltage, and, as needed, electrodes.
Methods and devices for simultaneous optical irradiation and oscillating magnetic field radiation of a target
An incubator-actuator device including a sample chamber, a magnetic field generating coil, and a light-emitting diode (LED) placement cage is provided herein. The incubator-actuator device is configured for simultaneous optical irradiation and oscillating magnetic field irradiation of a mammalian cell or a nanostructure. A system including an incubator-actuator device including a sample chamber, a magnetic field generating coil, and a light-emitting diode (LED) placement cage, and a laser is also provided herein. The system is configured for simultaneous optical irradiation and oscillating magnetic field irradiation of a target.
Methods and devices for simultaneous optical irradiation and oscillating magnetic field radiation of a target
An incubator-actuator device including a sample chamber, a magnetic field generating coil, and a light-emitting diode (LED) placement cage is provided herein. The incubator-actuator device is configured for simultaneous optical irradiation and oscillating magnetic field irradiation of a mammalian cell or a nanostructure. A system including an incubator-actuator device including a sample chamber, a magnetic field generating coil, and a light-emitting diode (LED) placement cage, and a laser is also provided herein. The system is configured for simultaneous optical irradiation and oscillating magnetic field irradiation of a target.
NANOSCREEN AND METHOD OF REGULATING STEM CELL ADHESION AND DIFFERENTIATION USING THE SAME
The present invention relates to a nanoscreen for regulating stem cell adhesion and differentiation. Moreover, the present invention relates to a method of regulating stem cell adhesion and differentiation using the nanoscreen. According to the nanoscreen of the present invention and the method of regulating stem cell adhesion and differentiation using the same, it is possible to efficiently regulate stem cell adhesion and differentiation by applying a magnetic field to the nanoscreen.
NANOSCREEN AND METHOD OF REGULATING STEM CELL ADHESION AND DIFFERENTIATION USING THE SAME
The present invention relates to a nanoscreen for regulating stem cell adhesion and differentiation. Moreover, the present invention relates to a method of regulating stem cell adhesion and differentiation using the nanoscreen. According to the nanoscreen of the present invention and the method of regulating stem cell adhesion and differentiation using the same, it is possible to efficiently regulate stem cell adhesion and differentiation by applying a magnetic field to the nanoscreen.
Well plate
A well plate comprises a plate main body and at least one cavity in an upper side of the plate main body. An upwardly open annular channel is formed in the at least one cavity, the annular channel being delimited at an inner circumference thereof by a closed circumferential wall. A horizontal outer circumference of the circumferential wall decreases from bottom to top up to an upper edge of the circumferential wall. Within the horizontal circumference of the circumferential wall, at its upper edge, at least two retaining elements connect upwardly to the upper edge of the circumferential wall. The at least two retaining elements are at a free horizontal distance to one another, and at least one of the at least two retaining elements is elastically supported at the plate main body in horizontal direction.