The invention discloses for the first time pluripotent cells, including hypoimmune pluripotent ABO blood type O Rhesus Factor negative (HIPO) cells, that evade rejection by the host allogeneic immune system and avoid blood antigen type rejection. The HIPO cells comprise reduced HLA-I and HLA-II expression, increased CD47 expression, and a universal blood group O Rh (O) blood type. The universal blood type is achieved by eliminating ABO blood group A and B antigents as well as eliminating Rh factor expression, or by starting with an O parent cell line. These new, novel HIPO cells evade host immune rejection because they have an impaired antigen presentation capacity, protection from innate immune clearance, and lack blood group rejection. The cells of the invention also include O pluripotent stem cells (iPSCO) and O embryonic stem cells (ESCO). The invention further provides universally acceptable off-the-shelf pluripotent cells and derivatives thereof for generating or regenerating specific tissues and organs.

The present invention provides a method for maintaining a continuous epithelial structure of a retinal tissue including culturing the retinal tissue in a medium comprising a methyl group donor or a substrate of the methyl group donor at a concentration at which cell differentiation of a neural retinal progenitor cell is suppressed, and a neurite extension inhibitor at a concentration at which neurite extension is suppressed.

A method for dynamic evolution and/or adaptation and monitoring of characteristics in living cells is provided, wherein the method may be performed at a microfluidic-enabled cell-culture device comprising pneumatic layer for directing flow of fluid to a plurality of individually addressable wells, and one or more sensors configured to detect data regarding environments inside one or more of the plurality of wells. The method may involve culturing a population of cells in a first well of the plurality of wells, perturbing one or more characteristics of an environment in the first well following the culturing of the population of cells, monitoring one or more characteristics of the population of cells in the first well, and removing all or part of the evolved/adapted population of cells from the first well.

The present invention falls within the technical field of cell repair, and specifically relates to a method for repairing stem cells and a use thereof. On the basis of the original invention technology, following improvement, in the present invention, pulse wave-treated water having the function of repairing stem cells is obtained by means of pulse wave-treated water having pulse wave sequences with equal widths and unequal intervals, and the method has additive effects on multiple treated samples. Without influencing cell proliferation, a culture system prepared from the pulse wave-treated water can not only reduce the natural apoptosis effect of human umbilical cord mesenchymal stem cells (H-MSC), but also can inhibit injury-induced H-MSC apoptosis, and has a cell repair effect.