The present invention relates to a method for isolating and culturing neural stem cells with high efficiency, which may shorten the time for isolation and culture by simplifying a method for isolating and culturing neural stem cells and may increase the acquisition yield of neural stem cells. The present invention provides a method for isolating and culturing neural stem cells with high efficiency, comprising the steps of adding brain tissue into an enzyme solution so as to subject the brain tissue to enzyme treatment; physically isolating cell clumps from the enzyme treated brain tissue by dividing the cell clumps according to size and removing impurities; and inoculating the cell clumps on a culture dish so as to subculture.

The present invention “A polypeptide EXP and its drug delivery system as well as extracellular vesicle extraction kit thereof” belongs to the field of biomedical engineering and diagnostics. The amino acid sequence of the polypeptide EXP is set forth in SEQ ID NO. 1. Based on the polypeptide EXP, the present invention also provides a drug delivery system, targeted drug delivery system, enhanced drug delivery vehicle, a drug with enhanced delivery, targeted drug, extracellular vesicle extraction kit, disease diagnostic kit, a method for purifying extracellular vesicles, and use of the polypeptide EXP in pharmacy and diagnostic reagent manufacture.

Provided are new methods for generating extracellular matrix material, compositions comprising the extracellular matrix material, and methods of using the extracellular matrix.

The present invention provides muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment soft tissue following introduction (e.g. via injection, transplantation, or implantation) into a site of soft tissue. Also provided are methods of isolating muscle-derived progenitor cells, and methods of genetically modifying the cells for gene transfer therapy. The invention further provides methods of using compositions comprising muscle-derived progenitor cells for the augmentation and bulking of mammalian, including human, soft tissues in the treatment of various cosmetic or functional conditions, including malformation, injury, weakness, disease, or dysfunction. In particular, the present invention provides treatments and amelioration of symptoms for gastro-esophageal pathologies like gastro-esophageal reflux.

Provided are a method of preparing synovium-derived mesenchymal stem cells using a synovial tissue and a hydrogel, synovium-derived mesenchymal stem cells prepared by the method, a pharmaceutical composition for treating bone or cartilage damage, the pharmaceutical composition including the synovium-derived mesenchymal stem cells, and a composition and kit for culturing mesenchymal stem cells, the composition and kit including a synovial tissue and a hydrogel. When the preparation method of the present invention is used, stem cells may be effectively extracted and obtained from synovium, and the obtained synovium-derived mesenchymal stem cells may effectively treat bone or cartilage damage, and therefore, it will be able to greatly contribute to the development of a method of treating bone or cartilage damage.

Use of an exosome derived from decidual natural killer (dNK) cells or a dNK cell subset in the preparation of a drug and an auxiliary therapeutic agent for infertility-related diseases is provided. Experiments have confirmed that the exosome treats the endometrial growth disorder-related diseases by promoting increase of endometrial thickness, increasing endometrial cell viability, reducing endometrial cell damage, promoting VEGF expression, and maintaining stemness and stimulating proliferation of endometrial stromal cells, such that a conception rate for endometrial injury model mice increases from 20% to 50%-70%; and the exosome treats the maternal-fetal immune tolerance disorder-related diseases by exerting immune tolerance, reducing a spontaneous abortion rate, and increasing a helper T cell level. In addition, the exosome effectively increases the development rate of eggs fertilized in vivo or in vitro in a multiplying way, and improves an implantation rate and a birth rate for in vitro fertilization and embryo transfer.

A closed-system (i.e., hermetically sealed) methods, and kits for isolating Mesenchymal Stromal Cells (MSCs) from lipoaspirate, that utilizes specific hermetically-sealed, disposable assemblies and containers that are aseptically interconnected, are disclosed. Contemplated methods require lipoaspirate as a starting material, and provide for obtaining isolated, purified MSCs at the end of the process. Kits are contemplated to contain disposable assemblies, composed by sterile components such as modified syringes, tubing, centrifuge tubes, filtering units, that can be aseptically connected while maintaining sterility thereby keeping the system closed (i.e., hermetically sealed) with respect to the external environment. As a result, contamination during the isolation process can be avoided. The MSCs that are obtained at the end of the processing are thus ready to be further manipulated in subsequent operations (in example, expansion) also for therapeutic purposes.

A cell culture instrument configured to detach cells at a desired position and a cell processing method using the cell culture instrument. The cell culture instrument includes: a substrate; and a photoreactive layer having a photosolubility and a photothermal convertibility, wherein the photoreactive layer is laminated on the substrate, and the photoreactive layer includes a polymer having a photosolubility and a photothermal convertibility.

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.

An extracellular vesicle isolation method using a metal, and a method for isolating extracellular vesicles from various samples using metal affinity are disclosed. An extracellular vesicle isolation method has the advantages of not requiring costly equipment, of being able to be applied without limits on sample quantity, and of being capable of efficiently isolating extracellular vesicles while preserving the shape or properties thereof. Moreover, the method can be combined with existing isolation methods to maximize the efficiency of extracellular vesicle isolation, and can be utilized in disease diagnosis, disease treatment, multi-omics research, and extracellular vesicle property research and the like using the isolated extracellular vesicles.