The present invention pertains to a cell culture medium comprising copper as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.

The present disclosure relates to eukaryotic cell lines with a stable integrated loss-of-function or attenuation-of-function mutation in each of the Bax and Bak genes. Also provided are methods of producing such cell lines. This disclosure also relates to compositions and cell cultures comprising such cell lines, as well as methods of producing a product, such as a recombinant polypeptide or viral vector, using said cells, compositions and cell cultures.

The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.

The present invention provides for a genetically modified bacterial host cell capable of producing indigoidine, wherein the host cell comprises a non-ribosomal peptide synthetase (NRPS) that converts glutamine to indigoidine, and the bacterial host cell is reduced in its expression of one or more of the sixteen indicated enzymes.

The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.

Provided herein are cell lines (e.g., HEK293 cell lines) that comprise a loss-of-function mutation in each of the human Bax and Bak genes, as well as cell cultures comprising the cell lines. The cell lines and/or cell cultures may find use, e.g., in methods for producing a recombinant polypeptide (such as an antibody or antigen-binding fragment thereof) or a viral vector.

The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

NKp46-binding immunoglobulin variable regions, and proteins such as antibodies and multispecific proteins that comprise the variable regions are provided. The proteins can bind and specifically redirect NK cells to lyse a target cell of interest. The proteins have utility in the treatment of disease, notably cancer or infectious disease.

The present invention is directed to a methods for the stimulation of hair, lash and/or nail growth by the administration of extracellular matrix (ECM) and/or conditioned culture media (CCM) compositions in combination with active agents such as minoxidil and bimatoprost. The combination of hypoxic CCM and an active agent acts to promote hair, lash and/or nail growth and/or promote hair follicle development and/or activate or stimulate on an area of the skin when administered to the region of skin or tissue in need of growth or repair in a subject.

The present application belongs to the field of biotechnology and pharmacy, and provides a fusion protein containing a fibronectin type III domain and preparation and application thereof. The structure of the fusion protein comprises a fibronectin type III domain and an insertion sequence, which can maintain the spatial conformation of an inserted protein or an active peptide, and can effectively protect the N-terminal and/or the C-terminal of a target protein, so that the target protein is not easily affected by enzymolysis, and thus obtaining a longer half-life in vivo.