Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.

Provided is a host cell for stably propagating a virulent hand, foot and mouth disease virus, the host cell expressing no heparan sulfate and overexpressing primate scavenger receptor class B member 2 (SCARB2). Also provided is a method for screening for an anti-hand, foot and mouth disease virus vaccine or an anti-hand, foot and mouth disease virus drug using a stably cultured virulent hand, foot and mouth disease virus.

Disclosed are mammalian cells and mammalian cell lines that have a reduced load of remnants of past viral/retroviral infections and methods of producing and using the same.

The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.

Methods of culturing and manufacturing of cells on a large-scale level are disclosed. Particularly, a manufacturing system and device, and methods of using the system and device for culturing and manufacturing cells in hollow fibers made from alginate polymers are provided.

Provided is a method for isolation and expansion of dermal papilla cells, and more particularly, to a method of isolating dermal papilla cells from hair bulbs, isolated from scalp tissue, by chopping, and then expanding the isolated dermal papilla cells by passaging. The expanded dermal papilla cells may play an important role in hair growth, and thus the present invention may be used in various industrial fields, including the medical field and the cosmetic field.

The invention provides methods and materials for culturing mammalian cells and harvesting recombinant protein.

The present invention relates to a new isolated strain of Pseudomonas sp. deposited at the “Colección Española de Cultivos Tipo” (CECT) under the accession number CECT8708 or a mutant thereof; wherein said mutant is obtained using the CECT8708 of Pseudomonas sp. as starting material, maintains the dual bactericidal and fungicidal effects of CECT8708 and has a genome with an Average Nucleotide Identity (ANI) to the genome sequence SEQ ID NO: 1 of at least 99.8%. The strain of the invention has bactericidal and fungicidal effect.

The invention also relates to the inoculation product comprising the strain of the invention, the cell-free extract derived from the strain of the invention, and the composition comprising it and their uses in the control of bacteria and/or fungal infection in a plant. Also refers to a method to obtain the mutant strain of the strain of the invention.

The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods.

Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.