By a method for producing a culture for obtaining a culture by culturing a lactic acid bacterium and/or a bacterium belonging to the genus Bifidobacterium in a milk peptide-containing medium which is characterized in that a culture temperature is 3° C. or more lower than an optimum culture temperature of the lactic acid bacterium and/or the bacterium belonging to the genus Bifidobacterium, the change in the acidity during the storage of the product is not accelerated, and the bacterial count at the time of the preparation of the product can be maintained at a high level, even when the lactic acid bacterium or the like is cultured in a medium containing a milk peptide added thereto.

The present invention relates to the use of warmers, or autonomous heat packs, for heating and maintaining a solution at a suitable temperature, for the period of time required to accomplish a chemical, biochemical or biological reaction, in particular in molecular biology or cell biology applications. Biology kits containing warmers are also part of this invention.

Compositions disclosed herein, and methods of use thereof included those for inhibiting or reducing the incidence of cytokine release syndrome or cytokine storm in a subject undergoing CAR T-cell therapy, methods of treating a cancer or tumor, methods of reducing tumor load, methods of reducing the size or growth rate of a cancer or a tumor, and methods of extending of the survival of a subject suffering from a cancer or tumor, wherein the subjects are administered compositions comprising apoptotic cells or apoptotic cell supernatants. Compositions and methods of use thereof may increase the efficacy of a CAR T-cell cancer therapy. Disclosed herein are also compositions and methods of use thereof for decreasing or inhibiting cytokine production in a subject experiencing cytokine release syndrome or cytokine storm. In certain instances compositions may include additional chemotherapeutic or immunomodulatory agents.

A Pseudomonas graminis strain capable of degrading cellulose at a low temperature and use thereof. The accession number of the Pseudomonas graminis strain is CGMCC NO.: 18751. The strain has relatively strong capability of degrading cellulose at both a low temperature of 4° C. and a condition of 10° C., and the cellulose degradation capacities are basically the same. Although the cellulose degradation capacity is weakened at 30° C., the degradation amplitude is not large, and meanwhile, a KY240 strain also has potassium and inorganicphosphorus degradation capacity, and is expected to be used for preparing a bacterial fertilizer for degrading cellulose, promoting straw decomposition and improving soil fertility, particularly improving the amount of potassium and phosphorus available for plants in soil.

A method and modified fermentation intermediate are disclosed for the production of polyunsaturated fatty acid (PUFA). The method comprises heat sterilizing a fermentation medium comprising dextrose to produce a heat sterilized fermentation medium, wherein the heat sterilizing converts at least a portion of the dextrose to DP2+ sugars. The method comprises combining the heat sterilized fermentation medium with an enzyme capable of converting DP2+ sugars to dextrose, thereby producing a modified heat sterilized fermentation medium comprising more dextrose and less DP+2 sugars than without combining the medium with the enzyme. The modified heat sterilized fermentation intermediate may be placed in contact with a microorganism to produce PUFA, wherein the microorganism is capable of utilizing dextrose to produce PUFA.

The invention provides a microfluidic device comprising at least one cell culture chamber, the at least one cell culture chamber being connected to at least two openings, the device being configured to supply at least one physiologically active substance from at least one of the openings to the at least one cell culture chamber in such a manner as to form a concentration gradient or concentration gradients in the at least one chamber when cells and a hydrogel are introduced into the at least one cell culture chamber to culture the cells in a 3D-gel medium.

The invention provides methods for manufacturing purified preparations of achromosomal dynamic active systems (ADAS), including highly active ADAS. These ADAS provided by the invention can be obtained by a variety of means. Various associated methods of making and using these ADAS are provided.

The present invention relates to a preparation method of a composition for culturing pancreatic organoids, a composition thereby, and an organoid culture method using the same. The present invention is capable of creating an environment that is more similar to an actual tissue than a conventional Matrigel-based culture system, and in particular, exhibits an effect of facilitating tissue differentiation in pancreatic organoid culture and effectively developing into a form that is similar to an actual tissue.

The present invention relates to the technical field of microbial fermentation engineering, and specifically to a method for producing psicose 3-epimerase by high-density fermentation. In view of the low expression level of psicose 3-epimerase and other problems existing in the current fermentation, by controlling the feeding rate in the fermentation process, improving the culture temperature in the middle and late stages of fermentation and other measures in the present invention, the OD value during the fermentation with recombinant Bacillus subtilis and the total enzyme activity of psicose 3-epimerase in the fermentation broth are significantly increased, the expression of psicose 3-epimerase is markedly improved, and the production cost of allulose is reduced. Therefore, the present invention has a very broad prospect of application in industry.

The present invention relates to bispecific chimeric polypeptide assembly compositions comprising bulking moieties linked to binding domains by cleavable release segments that, when cleaved are capable of concurrently binding effector T cells with targeted tumor or cancer cells and effecting cytolysis of the tumor cells or cancer cells. The invention also provides compositions and methods of making and using the cleavable chimeric polypeptide assembly compositions.