Cardiopulmonary progenitor exosomes and a preparation method thereof are provided. A culture medium of the cardiopulmonary progenitors is prepared by adopting a preparation method developed by an inventor aiming at the cardiopulmonary progenitors, the supernatant is taken, and the cardiopulmonary progenitor exosomes are isolated and extracted by ultracentrifugation. The applications of the cardiopulmonary progenitor exosomes in reducing the area of cardiac necrosis and fibrosis, promoting the improvement of cardiac function, the proliferation of cardiomyocytes and the angiogenesis of injured hearts are provided, which indicates that they have great potential in preventing and treating cardiovascular diseases.

The present invention relates to the expanding field of research relating to long-term living of humans in space environment, particularly to systems and processes for the production of high-quality, high-protein nutritional products, particularly for the production of cultured meat products in space.

A method and an automated liquid handling system for the isolation of particles from a biological sample are provided. A column, a container and a filtration unit which are adapted to be used in such a method and system are provided. The column can include a section comprising a plurality of microbeads retained there.

By swinging a culture vessel before aspiration removal of a medium, a centrally dense state (83) of the cell population is formed. In the centrally dense state (83), a cell cluster (84) is separated from an outlet port (58a). After aspiration removal of the medium, a new medium is introduced into the culture vessel. After the introduction of the new medium, an overall dispersed state of the cell population is formed.

A cultivation method for simulated microgravity-induced reprogramming of primary cancer cells and related culture media and reagents are in the field of biotechnology. A multi-directional G-force generator is used to simulate a 10.sup.3 g microgravity environment, inducing reprogramming of cancer/cancer-adjacent tissue cells derived from patients, maintaining stemness of primary cell populations, and achieving rapid, long-term, high-fidelity cultivation of tissue cells from patients. Using this cultivation method, 10.sup.6 cells can be obtained within 7 days from a 1 mm.sup.3 tissue block acquired during surgery for drug screening. Specially formulated culture media, washing solutions, and digestion solutions are also provided, effectively solving problems of contamination susceptibility and difficult digestion of clinical samples. The established cancer cell models maintain highly similar genetic characteristics to the original tissue and can reflect differences in drug sensitivity between different patients, providing a powerful tool for precision cancer treatment.

A method and an automated liquid handling system for the isolation of particles from a biological sample are provided. A column, a container and a filtration unit which are adapted to be used in such a method and system are provided. The column can include a section comprising a plurality of microbeads retained there.

Disclosed herein are systems and methods for testing effects of simulated microgravity on muscle structures. The system includes a clinostat and an adapter that is receivable into the clinostat. The adapter includes a housing that defines at least one receptacle. The system further includes an assembly having a substrate and a plurality of fibrils deposited on the substrate. The plurality of fibrils comprise collagen and extend along an axis. The assembly is receivable into a first receptacle of the at least one receptacle. The plurality of fibrils can have myoblasts thereon. Operation of the clinostat simulates microgravity. Test substances can screened using the muscle structures to determine effects on myogenesis.

A cultivation method for simulated microgravity-induced reprogramming of primary cancer cells and related culture media and reagents are in the field of biotechnology. A multi-directional G-force generator is used to simulate a 10.sup.3 g microgravity environment, inducing reprogramming of cancer/cancer-adjacent tissue cells derived from patients, maintaining stemness of primary cell populations, and achieving rapid, long-term, high-fidelity cultivation of tissue cells from patients. Using this cultivation method, 10.sup.6 cells can be obtained within 7 days from a 1 mm.sup.3 tissue block acquired during surgery for drug screening. Specially formulated culture media, washing solutions, and digestion solutions are also provided, effectively solving problems of contamination susceptibility and difficult digestion of clinical samples. The established cancer cell models maintain highly similar genetic characteristics to the original tissue and can reflect differences in drug sensitivity between different patients, providing a powerful tool for precision cancer treatment.