Disclosed herein are methods for generating satellite cells and compositions including satellite cells.

The present invention provides improved methods and media for culturing stem cells and propagating organoids. In particular, the methods provide improved methods for culturing normal and tumour or cancer stem cells and their propagation into organoids. Specifically provided are suspension methods with particular utility for scalable organoid expansion, biobanking, experimental manipulation, miniaturization and imaging based high-throughput drug screening.

The present invention relates to a process for depositing at least a culture of microorganisms to an elongated element, preferably a yarn, comprising the steps of: providing at least a first feeding device comprising at least a first outlet; supplying at least one elongated element to said at least first feeding device; feeding to said first outlet at least a first culture comprising at least one microorganism; dispensing said first culture from said at least first outlet; contacting at least part of said elongated element with said first culture of microorganisms, to provide at least a part of said elongated element with an amount of said first culture of microorganisms.

The present set of embodiments relate to a bioproduction system, method, and apparatus for creating a scalable bioreactor system. Specifically, the present set of embodiments enable the determination of bioreaction performance characteristics of a commercial scale by matching operational parameters between a small test scale bioreaction to that of a commercial scale bioreaction. The system and methods do not rely on simply making bioreactor apparatuses across scales the same dimensionally which would not account for differences in fluid dynamic properties between very small to very large volumes, but requires tuning of a variety of systems (mixing assembly, sparger system, and headspace airflow system) in conjunction with one another to achieve predictive outcomes.

The disclosure features methods and compositions for differentiating stem cells into hematopoietic stem and progenitor cells (HSPC) and/or Natural Killer (NK) cells. The methods and compositions described herein are used to differentiate stem or progenitor cells having at least one gene-edit that is maintained in the differentiated cell. Also provided are differentiated cells produced using the methods and compositions described herein for therapeutic applications.

A bioactive suspension derived from freshly disaggregated tissue is provided, as well as related methods of formulation and use. The bioactive suspension may comprise a cell-free supernate derived from epidermal and dermal tissue that has been enzymatically and mechanically disaggregated, then separated, and which may contain tissue regeneration factors known to speed healing. The bioactive suspension may further comprise genetically-modified treatment cells, wild type cells, or both, and may be combined with one or more scaffolding elements to form a bioactive suspension combination product suitable for treatment of a cutaneous defect. Synthetic bioactive suspensions and bioactive suspension combination products are also provided.

In some aspects and embodiments, the invention provides methods for making hematopoietic stem cells, including for HSCT. The method comprises providing a cell population comprising hemogenic endothelial (HE) or endothelial cells, and increasing activity or expression of DNA (cytosine-5-)-methyltransferase 3 beta (Dnmt3b) and/or GTPase IMAP Family Member 6 (Gimap6) in the HE and/or endothelial cells under conditions sufficient for stimulating formation of HSCs.

Described is a crosslinked hydrogel for muscle stem cell culture and a preparation method and use thereof. The preparation method includes: dissolving collagen to prepare a solution and adding alginate and heparan sulfate proteoglycan and uniformly mixing with the collagen solution; adding ε-PL and TGase into the solution, uniformly stirring, and putting a slurry into a mold for crosslinking to obtain the hydrogel. The hydrogel is prepared by linking the collagen, the polylysine, and the heparan sulfate proteoglycan using the TGase to form covalent crosslinking, and forming a compact three-dimensional “egg box” network structure through a physical electrostatic interaction between the polylysine and the alginate.

Provided herein are novel organoid culture media, organoid culture systems, and methods of culturing tumor organoids using the subject organoid culture media. Also provided herein are tumor organoids developed using such organoid culture systems, methods for assessing the clonal diversity of the tumor organoids, and methods for using such tumor organoids, for example, for tumor modelling and drug development applications. In particular embodiments, the tumor organoid culture media provided herein is substantially free of R-spondins (e.g., R-spondin1).

Provided is a method of culturing mesenchymal stem cells as a cell construct while maintaining undifferentiation thereof, the method including subjecting mesenchymal stem cells to shaking culture.