The disclosure provided herein relates generally to mesenchymal-like stem cells hES-T-MiSC or T-MSC and the method of producing the stem cells. The method comprises culturing embryonic stem cells under conditions that the embryonic stem cells develop through an intermediate differentiation of trophoblasts, and culturing the differentiated trophoblast in hES-T-MSC or T-MSC, T-MSC derived cells and cell lineages T-MSC-DL are also described. Disclosed also herein are solutions and pharmaceutical compositions comprising the T-MSC and/or T-MSC-DL, methods of making the T-MSC and T-MSC-DL, and methods of using the T-MSC and T-MSC-DL for treatment and prevention of diseases, specifically. T-MSC and T-MSC-DL are used as immunosuppressive agents to treat multiple sclerosis and autoimmune diseases.

The technology described herein relates, at least in part, to compositions comprising and methods for isolating and enriching natural IgM-producing phagocytic B (NIMPAB) cells and methods of producing IgM antibodies using such cells, as well as uses of the antibodies produced by the methods for the prevention and treatment of diseases wherein immunotherapy with such natural IgM antibodies and their derivatives can be useful.

Described herein are a system, device, methods and compositions related to generating 3-dimensional cardiac tissues. Also described herein are a system, device, and methods of maturing 3-dimensional cardiac tissues and maintaining their viability in culture.

A stem cell therapy using an energy field (e.g., a laser beam) to prime stem cells, and then using a matching energy field to direct the primed stem cells to a target tissue. Preferably, the matching energy field has the same frequency as the energy field use to prime the stem cells (with no more than 10 Hz variation). Stem cells can be primed ex vivo or in vivo, by being exposed to the energy field, and/or a growth hormone. The stem cell therapy can be used to treat diseases including heart disease, spinal cord injury, and neurodegenerative diseases.

The present invention relates to a method for preventing cancerization of normal cells due to low-dose irradiation. The method enables effective prevention of cancerization of normal cells by means of low-dose radiation which is innocuous to a subject.

The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.

The invention comprises depletive methods of sex-sorting sperm at high event rates, while simultaneously achieving high technical yields.

A well for electrically stimulating at least one cell. The well includes a bottom portion and comprises an adaptive electrode arrangement for introducing an electric field into the well. The adaptive electrode arrangement includes a pair of electrodes disposed within the well. Each electrode of the pair of electrodes has a distal end and is independently and axially displaceable relative to the other electrode and the bottom portion of the well. The distal end of each electrode of the pair of electrodes is in contact with the bottom portion of the well, ensuring a uniform and constant electric field is applied within the well.

In some embodiments provided herein is a method of preparing cargo-loaded platelets, comprising: treating platelets with a cargo and with a loading buffer comprising a salt, a base, a loading agent, and optionally ethanol, to form the cargo-loaded platelets.

In some embodiments provided herein is a method of preparing cargo-loaded platelets, comprising: treating platelets with a cargo and with a loading buffer comprising a salt, a base, a loading agent, and optionally ethanol, to form the cargo-loaded platelets.