Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.

Methods and devices are provided for the formation of cardiac tissue constructs. In some embodiments, methods are provided for forming cardiac tissue constructs that including cardiomyocytes, non-myocytes, and extracellular matrix, and which exhibit properties associated with healthy cardiac tissue. In some embodiments, microfabrication platforms are provided to support the transmission of dynamic electromechanical forces, such that the cardiac microtissue constructs may be formed mimicking the basic microenvironment found in the heart. The microfabrication platform may include retaining features for stabilizing the position of the microtissue construct during its formation, and the microfabrication platform may include a ramped support configured to produce tissue constructs having a ring geometry. In some embodiments, the microfabrication platform may be configured to for the application of point electrical stimulation, and/or to amplify the transduction of force into a visible displacement.

The present invention refers to a non-invasive method for isolating adipose-derived stem cells from adipose tissue by illuminating the adipose tissue with at least one laser having a wavelength of between 360 nm to 480 nm or between 800 nm to 2 pm for a predetermined time for dissociating fat cells and blood vessels of the adipose tissue to release intact stem cells from the adipose tissue while maintaining the viability of the stem cells. In the preferred embodiment, the adipose tissue is illuminated with pulsed near infra-red (NIR) laser and a continuous wave (CW) diode blue laser. The present invention also contains a system adapted for isolating adipose-derived stem cells from adipose tissue using the method of the present invention.

Methods of generating cardiomyocytes from induced pluripotent stem cells (IPSCs) are provided. More specifically, the present disclosure relates to methods of generating cardiomyocytes from iPSCs using electrical stimulation. In some aspects, uses of such cells for therapeutics and in methods of treatment are provided.

Platelet rich plasma containing human very small embryonic-like stem cells (hVSEL) is treated with amplitude-modulated pulses of laser light having a predefined wavelength for a predefined time period, where the predefined wavelength ranges from 300 nm to 1000 nm. Treatment of the platelet rich plasma using this method results in an unexpectedly high degree of proliferation of the hVSEL in the platelet rich plasma, resulting in reduction of biological age, and treatment of diseases, when administered to a patient.

A method and system for enhancing migration of precursor cells in a desired direction, comprising applying a biphasic monopolar electrical field to the precursor cells. The method and system can be used to treat injury or disease of neural or skin tissue.

Compositions and methods are provided for the generation of highly potent conditioned stem (Epinul) cells from adult somatic cells or tissues. Such conditioned stem cells are capable of generating all the cell lineages of any tissue or organ. Uses and compositions of the conditioned stem cells are also disclosed.

In vitro method for stem cell proliferation and use of a device for increasing the proliferation of stem cells in vitro

The invention relates to an in vitro method for the proliferation of stem cells, comprising a step in which the stem cell culture is treated with an alternating current with a frequency in the 0.4 MHz to 0.6 MHz range, and to the use of a device for generating alternating current with a frequency in the 0.4 MHz to 0.6 MHz range in order to increase the proliferation of stem cells in vitro.

The present invention relates to compositions that comprise monocytes that enhances the survival of stem cells and methods of transplanting such compositions for treating various conditions.

A well for electrically stimulating at least one cell. The well includes a bottom portion and comprises an adaptive electrode arrangement for introducing an electric field into the well. The adaptive electrode arrangement includes a pair of electrodes disposed within the well. Each electrode of the pair of electrodes has a distal end and is independently and axially displaceable relative to the other electrode and the bottom portion of the well. The distal end of each electrode of the pair of electrodes is in contact with the bottom portion of the well, ensuring a uniform and constant electric field is applied within the well.