The present invention relates to a method and an apparatus for in vitro three-dimensional cell co-culture, wherein said method comprises a step of seeding a plurality of cells of a first cell type on a first magnetic prismatic porous scaffold and a plurality of cells of a second cell type on a second magnetic prismatic porous scaffold, while keeping the first and second scaffolds physically separate, and a step of moving the first and second scaffolds towards each other under the action of a magnetic field generated by a magnetic field generator until contact occurs on at least one surface.

The present invention relates to a method for preparation of an immortalized stem cell line that has an immortalizing gene introduced thereinto and retains an expression potential of the introduced gene while being restrained from proliferation so that the immortalized stem cell line can be used as a cell therapeutic agent, and to a use thereof. The immortalized stem cell line of the present invention, which has undergone a radiation process, cannot proliferate while maintaining the expression of the introduced foreign protein at a certain level or higher and as such, can be advantageously used as clinical samples such as various cell therapeutic agents.

The present disclosure relates to a method for producing exosomes through electrical stimulation. More particularly, the present disclosure relates to a method for producing exosomes, the method including: (a) applying radio frequency (RF) electrical stimulation to cells and culturing the cells; and (b) isolating exosomes from the cells and a culture medium containing the cells.

A cardiac organoid containing 3-D matter of adult human heart tissue.

The present invention relates to a process for microbial synthesis process having less water consumption and yielding desired product profile. More particularly, the present invention relates to an improved process of microbial synthesis using ultra fine nutrient mist in a specially designed biofilm-bioreactor under controlled conditions. The present invention also relates to an apparatus for microbial synthesis and preparation of optimized biofilm for continuous product formation.

Methods for preparing antigen-loaded dendritic cells (DCs) for treatment of cancer in a subject are provided, using an electroporation apparatus having an electroporation chamber including an upper electrode, a lower electrode and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of DCs into the electroporation chamber and a first output allowing passage of antigen-loaded DCs from the electroporation chamber. The first input and the first output are separated by an offset distance. Methods of treating cancer by administering antigen-loaded DCs are also provided.

The present invention relates to a nanocoil-substrate complex for controlling adhesion and differentiation of stem cells, a manufacturing method thereof, and a method of controlling adhesion and differentiation of stem cells by using the nanocoil-substrate complex, and the method of controlling adhesion and differentiation of stem cells may temporally and reversibly control adhesion and phenotypic differentiation of stem cells in vivo and ex vivo by controlling application/non-application of a magnetic field to the nanocoil-substrate complex.

A method for culturing heart tissue by culturing a slice of the heart tissue in a culture medium, wherein the culture medium comprises fetal bovine serum (FBS), vascular endothelial cell growth factor (VEGF), and fibroblast growth factor (FGF); and applying electrical stimulation to the slice while the heart slice is in culture is provided. Methods of screening candidate therapeutic agents for therapeutic effect or cardiotoxicity using the culture system are also provided.

An apparatus for separation of cells in a liquid into subsets of cells comprises a microfluidic chip comprising: a microfluidic channel having a liquid inlet for receipt of a stream of cell containing fluid; a detection zone disposed in the microfluidic channel and comprising a sensor configured to detect changes in the microfluidic channel corresponding to cells passing the sensor; and a separation zone distal of the detection zone in which the microfluidic channel divides into at least two secondary microfluidic channels, the separation zone comprising two or more separation electrodes, wherein the two or more separation electrodes include at least one separation electrode disposed in electrical contact with an interior of the microfluidic channel and at least one further separation electrode disposed in electrical contact with an interior of the microfluidic channel or one of the secondary microfluidic channels.

Current invention relates to biotechnology sphere and particularly describes the novel approach for non-attached biofilm-like aggregates fabrication in vitro under artificial microgravity environment conditions. Non-attached aggregates are grown under magnetic levitation conditions achieved by placing microorganisms suspension in paramagnetic nutrient medium in inhomogeneous magnetic field created by specifically developed permanent magnets. The invention method can produce biofilm-like aggregates from protozoa, fungi, microalgae, Gram-positive or Gram-negative bacteria and/or a consortium. The presented technology can be used for development of medications for treatment of chronic infections, antiseptics and solutions for surfaces, as well as in other applications requiring the use of non-attached biofilm-like aggregates model.