Methods for preparing antigen-loaded dendritic cells (DCs) for treatment of cancer in a subject are provided, using an electroporation apparatus having an electroporation chamber including an upper electrode, a lower electrode and a path defined in the electroporation chamber. The electroporation apparatus includes a first input allowing passage of DCs into the electroporation chamber and a first output allowing passage of antigen-loaded DCs from the electroporation chamber. The first input and the first output are separated by an offset distance. Methods of treating cancer by administering antigen-loaded DCs are also provided.

Magnetic cells plus a magnet are used to treat ED and other bodily lesions, whereby the magnetic cells are held in at or near the location of the lesion with a magnetic field for a period of time until efficacy is established. The cells can be any cell type, including stem cells, autologous cells, recombinant cells, combinations thereof and the like.

A method for isolation and culturing of T Lymphocytes from whole blood and harvesting exosomes. The method comprises isolating peripheral blood mononuclear cells from human blood, culturing the isolated peripheral blood mononuclear cells, treating the cultured peripheral blood mononuclear cells with cold atmospheric plasma for secretion of microvesicles, and harvesting microvesicles from the CAP-treated peripheral blood mononuclear cells.

Provided herein are MRI agent-loaded platelets, methods of preparing MRI agent-loaded platelets, and methods of using MRI agent-loaded platelets. In some embodiments, methods of loading MRI agents into platelets include contacting platelets with an MRI agent, a cell penetrating peptide, and a loading buffer that can include a salt, a base, a loading agent, and optionally at least one organic solvent.

Described herein are methods and compositions for regulation of the p53 pathway, providing intrinsic “sternness” allowing for their efficient in vitro expansion following isolation. Pharmacologic approaches to modulate p53 signaling supports expansion of stem cells, including greater clonal expansion of lung stem cells when compared to use of other small molecules such as ROCK inhibitor Y27632 alone. Effects of combined treatment with Pifithrin-α and Y27632 are additive. The current invention involves use of drugs that target the p53 pathway to reversibly regulate stem cell expansion in vitro for banking of stem cells and for pre-conditioning of stem cells prior to orthotopic transplantation.

The present invention relates to a method for transdifferentiating cells. The present invention provides a method for transdifferentiating fibroblasts into chondrocytes, comprising: forming a micromass of fibroblasts by culturing fibroblasts in a high density; and applying an electrical stimulation such as a current or magnetic field to the micromass of fibroblasts while culturing the micromass of fibroblasts in a culture medium not containing growth factors.

Compositions and methods are provided for the generation of highly potent conditioned stem (Epinul) cells from adult somatic cells or tissues. Such conditioned stem cells are capable of generating all the cell lineages of any tissue or organ. Uses and compositions of the conditioned stem cells are also disclosed.

The invention relates to a method for preparing an irradiated platelet lysate, comprising the steps providing a platelet lysate to obtain a starting platelet lysate, the starting platelet lysate having platelet factors including growth factors and having plasma proteins including coagulation factors and proteins other than the coagulation factors. Irradiating the starting platelet lysate using UVC radiation with a wavelength of between 200 and 280 nm, in order to obtain a platelet lysate irradiated with UVC radiation. The irradiation being arranged to retain at least 75% of the total protein concentration of the starting platelet lysate, while reducing, by at least 20%, the concentration of at least one of the coagulation factors including fibrinogen, factor II, factor VII, factor IX, factor X and factor XI of the starting platelet lysate.

Differentiation of a stem cell involves arranging the stem cell on a scaffold having an ordered array of magnetic one-dimensional nanostructures and culturing the stem cell while applying a magnetic field having a frequency to the ordered array of magnetic one-dimensional nanostructures to differentiate the stem cell.

A composition of matter for tissue engineering is disclosed. The composition comprises a plurality of electrospun albumin fibers, wherein an outer surface of the composition comprises a pattern of ridges or indentations, wherein the ridges or indentations are wider than the diameter of a single electrospun albumin fiber of the plurality of electrospun albumin fibers.