A composition including adult pluripotent olfactory stem cells is provided. The adult pluripotent olfactory stem cells are obtained by culturing a cell mixture from an olfactory tissue of a mammal in media containing growth factors and then isolating cells which express B-lymphoma moloney murine leukemia virus insertion region-1 (Bmi-1).

The invention relates to a method for activating a surface by increasing the hydrophilicity and/or for binding target structures, particularly selected from the group consisting of proteins, cellular proteins, proteins, cells, carbohydrates, peptides and amino acids. It also relates to the use of an oxidoreductase for activating a surface for the mentioned bonds and to corresponding activated surfaces.

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

A multiscale hierarchical scaffold with the complexity of extracellular matrix (ECM) is disclosed. The multiscale hierarchical scaffold may be a carbon-based scaffold with hierarchical nanoscale and microscale architecture with controlled physicochemical properties. More specifically, multiscale hierarchy is built by growing carbon nanotube carpets on two types of scaffolds, namely, interconnected microporous carbon foams and aligned carbon fiber mats.

A molecular delivery system including a plurality of nanowires (e.g., Si NWs), each of the nanowires having a surface layer formed of a silicon-containing material and a covalently bound linker (e.g., silane linker) attached to the surface layer and optionally including a substrate to which the nanowires are adhered or a molecule to be delivered attached to the linker. Also disclosed is a method of delivering into a cell an exogenous molecule.

Self-aligned fibrous scaffolds are disclosed. The scaffolds are capable of automechanoinduction of cell cultures and methods to induce authomechanoinduction in cancer cells and stem cells are disclosed as well.

The present invention generally relates to nanoscale wires and tissue engineering. Systems and methods are provided in various embodiments for preparing cell scaffolds that can be used for growing cells or tissues, where the cell scaffolds comprise nanoscale wires. In some cases, the nanoscale wires can be connected to electronic circuits extending externally of the cell scaffold. Such cell scaffolds can be used to grow cells or tissues which can be determined and/or controlled at very high resolutions, due to the presence of the nanoscale wires, and such cell scaffolds will find use in a wide variety of novel applications, including applications in tissue engineering, prosthetics, pacemakers, implants, or the like. This approach thus allows for the creation of fundamentally new types of functionalized cells and tissues, due to the high degree of electronic control offered by the nanoscale wires and electronic circuits.

Provided is a culture substrate having a periodic fine structure in the order of micrometers and a periodic fine structure in the order of nanometers on the same surface where stem cells are to be cultured on the surface.

Provided is a scaffold material for cell culture with which the culture stability of cells can be maintained over an extended period of time. A scaffold material for cell culture according to the present invention contains a peptide-conjugated (meth)acrylic copolymer having a (meth)acrylic copolymer moiety and a peptide moiety bonded to the (meth)acrylic copolymer moiety, the following relaxation time (CS.sub.2) being 5 milliseconds or less. Relaxation time (CS.sub.2): a relaxation time of a CS component, which is a component having the lowest molecular mobility among three components, when the scaffold material for cell culture after being brought into contact with heavy water at 25 C. for 18 hours is measured by a CPMG method using pulsed NMR, and the obtained free induction decay curve of spin-spin relaxation of .sup.1H is separated into three components.

Provided is a coating solution for forming a cell scaffold with which a cell scaffold having a large thickness can be easily formed and the proliferation of cells can be enhanced. A coating solution for forming a cell scaffold according to the present invention contains a peptide-conjugated resin having a synthetic resin moiety and a peptide moiety and an alcohol solvent, a content of the peptide-conjugated resin being 0.1 wt % or more.