The present invention provides a scaffold for culturing retinal tissue comprising an amount of gelatin, an amount of chondroitin sulfate, an amount of hyaluronic acid, wherein the amount of gelatin, chondroitin sulfate, and hyaluronic acid are prepared into a three-dimensional monolith, wherein the monolith is sectioned into planar sheets, and an amount of laminin-521.

3D native tissue-derived scaffolding materials are made in various formats, including but not limited to hydrogel, sponge, fibers, microspheres, and films, all of which function to better preserve natural extracellular matrix molecules and to recapitulate the natural tissue environment, thereby effectively guiding tissue regeneration. Tissue-derived scaffolds are prepared by incorporating a homogenized tissue-derived suspension into a polymeric solution of synthetic, natural, or hybrid polymers. Such tissue-derived scaffolds and scaffolding materials have a variety of utilities, including: the creation of 3D tissue models such as skin, bone, liver, pancreas, lung, and so on; facilitation of studies on cell-matrix interactions; and the fabrication of implantable scaffolding materials for guided tissue formation in vivo. The tissue-derived scaffolds and scaffolding materials also provide the opportunity to correlate the functions of extracellular matrix with tissue regeneration and cancer metastasis, for example.

The present invention relates to a process for depositing at least a culture of microorganisms to an elongated element, preferably a yarn, comprising the steps of: providing at least a first feeding device comprising at least a first outlet; supplying at least one elongated element to said at least first feeding device; feeding to said first outlet at least a first culture comprising at least one microorganism; dispensing said first culture from said at least first outlet; contacting at least part of said elongated element with said first culture of microorganisms, to provide at least a part of said elongated element with an amount of said first culture of microorganisms.

A method of efficiently recovering a large amount of exosomes from mesenchymal stem cells is provided. The method includes: a three dimensional culture step of three dimensionally culturing mesenchymal stem cells in a medium containing sugar by using a nonwoven fabric as a scaffold; a post-plateau culture step of further culturing for a certain period of time after the amount of the sugar consumed by the mesenchymal stem cells reaches a plateau; and an exosome recovery step of recovering exosomes from the mesenchymal stem cells. The mesenchymal stem cells are adipose-derived mesenchymal stem cells.

Implantable scaffolds that treat solid tumors and escape variants and that provide effective vaccinations against cancer recurrence are described. The scaffolds include genetically-reprogrammed lymphocytes and a lymphocyte-activating moiety.

A cellular support system comprises a three-dimensional scaffold structure comprising at least one void. At least one suspended protein bridge spans across the at least one void in the three-dimensional scaffold structure. The suspended protein bridge is capable of supporting cells and promotes three-dimensional cellular growth. In certain aspects, the protein in the suspended protein bridge is an extracellular matrix protein, such as collagens, laminins, fibronectins, and combinations thereof. Such a cellular support system supports thriving cell cultures in three-dimensions emulating cell growth in vivo in an extracellular matrix, including promoting cell remodeling. Methods for making such cellular support systems are also provided.

Provided herein are methods for the differentiation of pluripotent stem cells to committed cardiac progenitor cells. Further provided herein are methods for the use of the committed cardiac progenitor cells in the treatment of cardiac disorders.

This invention relates, e.g., to a Myocyte-based Flow Assist Device (MFAD) for treating a subject in need of increased flow of a biological fluid, such as venous blood or lymph, comprising a sheath which comprises rhythmically contracting myocytes.

The present invention relates to an in vitro method for creating a viable connective tissue and/or osseous tissue obtained by tribological solicitations of a biological culture. It further relates to a viable connective tissue and/or osseous tissue susceptible to be obtained by said method as well as to the use of said method or viable connective tissue and/or osseous tissue to prepare a biological implant.

A method for generating a population of cardiomyocytes from induced pluripotent stem cells (iPSCs) in an integrated process. The method may comprise seeding the iPSCs on a modified surface of a modified cell culture substrate, culturing the seeded iPSCs on the modified surface of the modified cell culture substrate in an animal component-free culture medium, and differentiating the cultured iPSCs to the population of cardiomyocytes on the modified surface of the modified cell culture substrate. The modified cell culture substrate may comprise a patterned polydimethylsiloxane (PDMS) substrate, a first coating comprising a plurality of polydopamine molecules, and a second coating comprising a plurality of Laminin 511 E8 Fragment (LME8) molecules.