The disclosure provides three-dimensional cross-linked scaffolds generated from peripheral blood plasma, and methods for making and using such scaffolds.

The disclosure provides three-dimensional cross-linked scaffolds generated from cord blood plasma, and methods for making and using such scaffolds.

Disclosed herein are organoid compositions that are manipulated to form shaped or elongated morphologies that more closely resemble native organ structures. These shaped organoids are advantageous for purposes such as studying organellar organization and for transplants compared to unformed organoids. Also disclosed herein are methods of producing said shaped or elongated organoid compositions.

A cell culture kit is provided. The cell culture kit allows a cultured cell to be easily separated when the cell is cultured and separates a cell without separate trypsinization for subculture.

Provided is a method for manufacturing cell-containing blocks having steps of: preparing an standardized size mold by 3D printing (three dimensional printing) using a biocompatible elastic material; injecting a thermosensitive colloid into the mold to form a thermosensitive mold; injecting a hydrogel containing cells in to the thermosensitive mold and curing the hydrogel containing cells to form the cell-containing blocks; separating the thermosensitive mold and the cell-containing blocks at a temperature higher than a solidifying point of the thermosensitive colloid. Also provided are method for assembling the cell-containing blocks in a target configuration by using an assembling mold defining the target configuration and made of a thermoreversible material.

A packed-bed bioreactor system for culturing cells is provided, the system including a cell culture vessel having at least one interior reservoir, an inlet fluidly connected to the reservoir, and an outlet fluidly connected to the reservoir; and a cell culture matrix disposed in the reservoir. The cell culture matrix includes a structurally defined multi-layered substrate for adhering cells thereto, and each layer of the multi-layered substrate has a physical structure and a porosity that are substantially regular and uniform.

Described herein are methods, compositions, and kits for forming engineered in vitro biomimetic, three-dimensional, tubular organoid structures by directed differentiation of human pluripotent stem cells within tubular channels formed in a hydrogel.

The present disclosure relates to compositions and methods for culturing a population of hepatocytes in vitro, comprising co-culturing the population of hepatocytes with at least one non-parenchymal cell population and incubating the co-culture in culture medium, wherein the co-culture is periodically incubated in culture medium that does not comprise serum (serum-free culture medium).

Methods fabricate an endothelialized myocardium usable for screening of a drug. A microfibrous hydrogel scaffold is manufactured with additive manufacturing that concurrently bioprints endothelial cells directly within the microfibrous hydrogel scaffold. A bioink is bioprinted into an arrangement of one or more microfibers. The bioink includes at least one crosslinking component and suspended endothelial cells. The crosslinking component or components are crosslinked to yield the microfibrous hydrogel scaffold having the endothelial cells embedded directly within. The microfibrous hydrogel scaffold is seeded with cardiomyocytes to yield the endothelialized myocardium with a controlled anisotropy. The endothelialized myocardium can be incubated until the endothelialized myocardium matures into spontaneously beating myocardial tissue having contractions aligned with the controlled anisotropy. The beating myocardial tissue can be used to screen a drug when the beating myocardial tissue is embedded within a microfluidic perfusion bioreactor.

The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.