A method and device of creating a lumen model in a microwell plate defining a well is provided. A rod is inserted through the well of the microwell plate and the well is filled with a polymerizable material. The material is polymerized in the well. The rod is removed from the well of the microwell plate such that the polymerized material defines a lumen.

The cell structure fixing and removing system includes: an alignment base made of a silicone resin, the alignment base including a flat sticking surface to which needles with skewed cell aggregates are to be stuck; and a cell structure removing tool placed on the sticking surface between the alignment base and the cell aggregate, the cell structure removing tool including: a frame extending to correspond to an outline of the sticking surface, the frame including a puncture opening in middle, a bottom surface of the frame forming an alignment surface parallel to the sticking surface; and a porous sheet stretched and arranged on the alignment surface to close the puncture opening of the frame, the porous sheet allowing penetration by the needle, wherein an outline edge of the puncture opening in the alignment surface of the frame of the cell structure removing tool is located in the outline of the sticking surface.

Disclosed herein are substrates for cell delivery to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide seeded cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

The present disclosure aims to provide a method of efficiently manufacturing a cell mass, a cell structure, or a three-dimensional tissue body using a culturing surface coated with a temperature-responsive polymer or a temperature-responsive polymer composition. The manufacturing method of a cell mass, a cell structure, or a three-dimensional tissue body of the present disclosure includes seeding and culturing cells on a culturing surface coated with a temperature-responsive polymer or a temperature-responsive polymer composition.

The present disclosure aims to provide a manufacturing method of a cell structure. The manufacturing method comprises producing a coated region in which a culturing surface is coated with a temperature-responsive polymer or a temperature-responsive polymer composition, forming a droplet of a cell suspension in the coated region, and performing cell culturing in the droplet. A surface zeta potential of the coated region is 0 mV to 50 mV.

Disclosed are methods of assessing the ability of a candidate therapeutic agent to reverse, reduce or prevent intestinal injury by a potential toxic agent using a three-dimensional, engineered, bioprinted, biological intestinal tissue model. Also disclosed are methods of assessing the effect of an agent on intestinal function, the method comprising contacting the agent with a three-dimensional, engineered, bioprinted, biological intestinal tissue model.

A method for in-vitro tissue cultivation. The method may include providing a seeded-scaffold to a scaffold holder suspended in a medium contained in a bioreactor chamber. The method may further include rotating, via a rotation mechanism which the bioreactor chamber is coupled to, the bioreactor chamber about two orthogonal axes based on a predetermined motion cycle as a stimulation for tissue growth. The method may further include applying, via a stimulator coupled to the bioreactor chamber, at least one other stimulation for tissue growth to the seeded-scaffold. An apparatus for in-vitro tissue cultivation.

The present invention relates to an in vitroprocess for preparing cell and tissue models comprising at least one step of culturing fibroblasts of the dermo-hypodermic junction; the models obtained and also the implementation thereof in processes for screening active agents that promote the differentiation of fibroblasts of the dermo-hypodermic junction into adipocyte, osteoblast and/or chondroblast cells.

Provided herein are methods of generating hindbrain cells, including respiratory hindbrain cells, from pluripotent stem cells. Also provided are methods of generating a three-dimensional organoid comprising a population of hindbrain cells including a heterogeneous population of interneurons.

A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.