The present disclosure relates to a microcarrier for cell culture comprising: polystyrene-based particles containing at least one or more of hydrocarbon oil having 12 or more carbon atoms, or pores derived therefrom, a method for producing the same, and a cell culture composition using the same.

The present invention is directed to improved methods of preparing cells and compositions for therapeutic uses.

Provided are a method for producing a hepatocyte culture and a method for improving or maintaining a function of a hepatocyte or a hepatic progenitor cell, each method including a culturing step of culturing a hepatocyte or a hepatic progenitor cell inside at least a part of a plurality of opening pores and communication pores of a porous film having the plurality of opening pores provided on a surface thereof and the communication pores for communicating the opening pores adjacent to each other; and a cell-containing porous film.

The invention relates to the fields of biomaterials, tissue engineering and regenerative medicine. More specifically, it relates to biomaterial substrates having precise surface properties and the use thereof to investigate cell-material interactions. Provided is a cell culture system having a biomaterial substrate which has at least a first linear surface gradient oriented orthogonally to a second linear surface gradient, wherein the first gradient and the second gradient are selected from the group consisting of stiffness (S), (aligned) topography (T) and wettability (W). Also provided is a cell screening platform having a combination of at least two, preferably at least three, more preferably four distinct cell culture systems.

Described herein are methods, compositions, and kits for directed differentiation of human pluripotent stem cells, neuromesodermal progenitors, and neural stem cells into biomimetic neural tissues comprising one or more rosette structures. Preferably, the methods provided herein direct differentiation of human pluripotent stem cells, neuromesodermal progenitors, and neural stem cells into biomimetic neural tissues comprising a singular neural rosette structure that is comparable to at least a portion of the developing human neural tube. Also described are engineered neural tissue preparations comprising biomimetic neural tissues comprising a singular rosette structure having regional neural progenitor phenotypes.

In an aspect, compositions, methods, and devices described herein provide a safer platform for the ex vivo expansion of cells for immunotherapeutic purposes that eradicates the possibility of having activating substrate transferred into the patient while maintaining and improving upon the level of cell activation and proliferation.

Nanofiber structures are provided as well as methods of use thereof and methods of making.

A three-dimensional culture method includes: providing a cell suspension, the cell suspension containing a cell (12C) and a culture medium (14M); providing a solid surface (10S), the solid surface having a plurality of raised portions (10Sp) whose height is not less than 10 nm and not more than 1 mm; attaching a liquid drop (16D) of the cell suspension to the solid surface (10S); and culturing the cell (12C) in the liquid drop (16D) under such conditions that a direction of gravity exerted on the liquid drop (16D) is toward the solid surface (10S).

A conductive porous fabric can be formed, such as by using a template material. The porous fabric can be conductive, such as thick enough to be self-supporting, or supported such as by another structure. The porous fabric can be used in implantable or percutaneous applications, such as to provide an immunoisolation barrier. In another example, the fabric can be coupled to an electric potential, such as to facilitate gas evolution when the porous fabric is located in an aqueous medium. Such gas evolution can be used for various purposes, such as to maintain living cell viability by providing oxygen, or for self-cleaning. Illustrative examples of porous fabric materials include gold, platinum, palladium, iridium, niobium, or a form of carbon such as graphene.

Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and/or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and/or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.