Described are decellularized human extracellular matrix (ECM) morsels for use in tissue regeneration and repair.

The present disclosure provides a method of fabricating curvature-defined (C-D) or shape-defined (S-D) concave and convex polydimethylsiloxane (PDMS) surfaces and a method of fabricating C-D or S-D convex and concave gel surfaces for use in cell and tissue culturing and in other surface and interface applications, and provides a method of using C-D or S-D convex and concave surfaces with varying curvatures to direct cell attachment, spreading, and migration.

Described herein is a beads-free bioprocessor as an automated and cost-effective T cell processing and manufacturing platform. T cells are a core component in CAR T cell therapies for cancer treatment, but are difficult to manufacture to scale in clinically relevant quantities. The 3D bioprocessor provides an alternative device that is scalable, beads-free, easy-to-use, and cost-effective for using CAR T cell therapy in cancer immunotherapy. Besides CAR T cell application, this platform technology has potential for many other applications such as cancer cell isolation.

A plant fat-based scaffold for growing cell-based meat products for consumption. The scaffold comprises primarily plant fats or waxes in addition to cell binding proteins and optional additional components that assist in the growth of cultivated animal cells. The scaffold can exist in both a liquified state during sterilization and a solid state during the formation of the scaffold, the seeding of the cultivated cells, and the cellular growth phase. The scaffold is capable of remaining in the final product for consumption or is partially or completely melted out of the final product and recycled into raw material for forming new scaffolds.

This invention provides a cell culture substrate comprising on its surface a fluorine-containing polymer that enables three-dimensional tissue culture. The cell culture substrate of the invention has a surface at least a part of which is composed of a resin composition comprising a fluorine-containing polymer having one or more fluorine atoms in a repeating unit and exhibits the oxygen gas permeability of 219 cm.sup.3 (STP)/(m.sup.2.Math.24 h.Math.atm) or higher. Three-dimensional tissue can be formed via cell culture with the use of the cell culture substrate of the invention.

The present invention is to provide a cell culture substrate including a block polymer including a segment having a lower critical solution temperature and a hydrophobic segment, the cell culture substrate further including an adhesive matrix, in which the adhesive matrix is an extracellular matrix and/or an adhesive synthetic matrix. Furthermore, the invention is to provide a cell culture substrate in which the extracellular matrix is at least one selected from laminin, fibronectin, vitronectin, cadherin, and fragments thereof, and/or the adhesive synthetic matrix is poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide] or an oligopeptide-carrying polymer.

Provided is a membrane for cell culture and differentiation. The membrane comprises a base portion and an array of protrusions consisting of a plurality of protrusions. The protrusions are substantially evenly distributed on the base portion. The plurality of protrusions has dimensions on the order of micrometers. In particular, the membrane consists of particles of different particle sizes of two or more types. One type of particles has an average particle size of 1 μm to 50 μm. Two or more types of particles of different particle sizes include nanoscale particles, 10-900 nm. One type of particle is selected from the group consisting of inorganic compound microspheres. The other type of particles of the two or more types of particles of different particle sizes is selected from the group consisting of organic polymer nanospheres. Also provided is a method for maintaining, culturing and/or differentiating cells using such membrane.

Embodiments of the disclosure provide a dissolvable or degradable artificial circulation system for engineering, culturing, and integrating large volume tissues. Also provided are methods of using large engineered tissues prepared using the degradable artificial circulation system for clinical applications and for various applications such as large-scale production of therapeutic or consumable products, drug discovery, and toxicity screening.

A polymerizable unit that yields an electrochemically responsive polymer (advantageously pyrrole) is anchored by polymerization within a polycaprolactone matrix to form an electroactive scaffold upon which cells can be cultured and in which the micro- and nano-topological features of the polycaprolactone matrix are preserved. A scaffold manufactured in accordance with the preferred embodiment can support Schwann cells, which produce nerve growth factor when electrically stimulated. Nerve growth factor has been demonstrated to promote the regeneration of nerve tissue. By implanting the scaffold on which Schwann cells have been cultured into damaged nerve tissue and applying a voltage across the scaffold, nerve growth factor is produced, thereby promoting repair of the damaged nerve tissue.

The present application provides a method for preparing transplantable cell preparation, the method comprising culturing eukaryotic cells at conditions allowing the cells to coalesce and form cell aggregates, providing the cell aggregates in a nanofibrillar cellulose hydrogel to obtain a transplantable cell composition comprising eukaryotic cells in a nanofibrillar cellulose hydrogel matrix, and a transplantable cell preparation. The present application also provides the transplantable cell composition for use in a therapeutic method, and to use of nanofibrillar cellulose for preparing the transplantable cell composition.