This nitrogen recovery method is for causing nitrifying bacteria to decompose an ammonia component in an ammonia-containing gas, and recovering a nitrogen component contained in ammonia as an ammonia gas decomposition product, involving: supplying circulating water to a microorganism decomposition tank retaining a nitrifying bacterium carrier carrying nitrifying bacteria to maintain the carrier wet; passing ammonia-containing gas through the carrier in the wet state in an oxygen-containing atmosphere; dissolving an ammonia component in the ammonia-containing gas in the circulating water, together with an ammonia gas decomposition product produced by the nitrifying bacteria, to continue decomposing the ammonia-containing gas while the decomposition product is accumulated in the circulating water; and collecting all or a portion of the circulating water to recover the ammonia gas decomposition product, when the concentration of nitrate ion as an ammonia decomposition product in the circulating water reaches a predetermined concentration of 5000 mg/L or more.

This nitrogen recovery method is for causing nitrifying bacteria to decompose an ammonia component in an ammonia-containing gas, and recovering a nitrogen component contained in ammonia as an ammonia gas decomposition product, involving: supplying circulating water to a microorganism decomposition tank retaining a nitrifying bacterium carrier carrying nitrifying bacteria to maintain the carrier wet; passing ammonia-containing gas through the carrier in the wet state in an oxygen-containing atmosphere; dissolving an ammonia component in the ammonia-containing gas in the circulating water, together with an ammonia gas decomposition product produced by the nitrifying bacteria, to continue decomposing the ammonia-containing gas while the decomposition product is accumulated in the circulating water; and collecting all or a portion of the circulating water to recover the ammonia gas decomposition product, when the concentration of nitrate ion as an ammonia decomposition product in the circulating water reaches a predetermined concentration of 5000 mg/L or more.

Methods and systems to control flexible gas fermentation platforms for improved conversion of CO.sub.2 into products is developed and particularly relates to a control process and system to control a ratio of feedstock gases and maximize the concentration of inert components in a bioreactor tail gas stream and or bioreactor headspace. Improved carbon utilization results though providing the most beneficial ratio of substrates to the bioreactor of the fermentation process.

A variety of methods and systems for screening biocatalysts are disclosed, including, in one embodiment, a screening method for identifying engineered biocatalysts, including reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons; reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons; and measuring activity of the engineered biocatalyst.

A variety of methods and systems for screening biocatalysts are disclosed, including, in one embodiment, a screening method for identifying engineered biocatalysts, including reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons; reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons; and measuring activity of the engineered biocatalyst.

Microorganisms present within a plurality of microorganism clusters immobilized in a porous support material may collectively define a supported bio-catalyst. When the microorganisms are effective to convert methane into one or more oxidized carbon compounds (e.g., methanotrophic bacteria), the supported bio-catalysts may be utilized to remove methane from methane-containing gas streams, such as those obtained from mining ventilation. Methods for processing a methane-containing gas stream may comprise interacting the gas stream with the supported bio-catalyst in substantial absence of a liquid phase, and obtaining a methane-depleted gas stream downstream from the supported bio-catalyst. Systems for processing a methane-containing gas stream may comprise the supported bio-catalysts housed in one or more vessels fluidly coupled to a source of methane-containing gas stream. A gas concentration in the methane-containing gas stream and/or the methane-depleted gas stream may be used to determine a current state or anticipated remaining lifetime of the supported bio-catalyst.

The present disclosure provides a Klebsiella pneumoniae Y7-3 with a deposit number of CCTCC NO: M2019851. The strain can degrade corn stover into acetic acid, ethanol, and hydrogen, and can further metabolize into acetic acid, ethanol, 1,3-propanediol, lactic acid, and hydrogen.

The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

The invention is directed to kits, compositions, tools and methods comprising a cyclic industrial process to form biocement. In particular, the invention is directed to materials and methods for decomposing calcium carbonate into calcium oxide and carbon dioxide at an elevated temperature, reacting calcium oxide with ammonium chloride to form calcium chloride, water, and ammonia gas; and reacting ammonia gas and carbon dioxide at high pressure to form urea and water, which are then utilized to form biocement. This cyclic process can be achieved by combining industrial processes with the resulting product as biocement. The process may involve retention of calcium carbonate currently utilized in the manufacture of Portland Cement.