Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

A method for site-selective deuteration of amino acids using a protein system having an aminotransferase (e.g., DsaD) and/or a small partner protein (e.g., DsaE). A non-deuterated amino acid is contacted with deuterium and an aminotransferase or a combination of an aminotransferase and a partner protein, to yield a Cα-deuterated or a Cα- and Cβ-deuterated amino acid. Cβ-deuterated amino acids can be accessed by contacting a Cα- and Cβ-deuterated amino acid with non-deuterium hydrogen and an aminotransferase to wash out the deuterium at the Ca carbon atom by the non-deuterium hydrogen.

The invention relates to a method and a device device for converting at least one reactant(5) into a reaction product and separating the at least one reactant from the reaction product, wherein the device comprises a vessel(10) with a vessel inner volume (11) and a confinement (20) submerged in the vessel inner volume (11), the confinement (20) providing a confinement inner (21) volume which is in fluid connection with the vessel inner volume (11), wherein the vessel inner volume (11) contains a first fluid (1) with a first density p1 and a second fluid with a second density p2, with p1 > p2, so that the first fluid (1) forms a lower phase and the second fluid (2) forms an upper phase in the vessel inner volume (11), wherein the confinement contains a third fluid (3) with a third density p3 with p3 > p2 so that the second fluid forms an upper layer and the third fluid forms a lower layer in the confinement inner volume (21), wherein the third fluid may be the same as or different from and is physically separated from the first fluid (1), wherein at least one of the first, second fluid and third fluid is at most partly with the other two, but preferably immiscible, wherein the at least one reactant (5) and the reaction product (6) have a different affinity for at least two of the first, second (2) and third fluid, wherein at least one of the first (1) and third fluid (3) contain a fourth phase (4) which is a solid or semi solid and is selected from the group of materials capable of promoting the conversion of the at least one reactant into the reaction product.

Provided is a method for synthesizing a chiral diamine compound. The synthesizing method includes: converting a substrate represented by Formula I into a chiral diamine compound in Formula I by using a transaminase, herein n=1˜10, an R group represents an alkyl, a cycloalkyl, a heteroatom-containing alkyl, a heteroatom-containing cycloalkyl, a heteroatom-containing aryl, an amide compound residue, or an ether compound residue, and a hetero atom is at least one from among O, S and N; R1 and R2 are the same or not the same, the R1 and R2 are respectively and independently hydrogen, a C1-C3 alkyl, or an amino protecting group; and the transaminases are derived from a plurality of strains.

A production equipment of phenethylamine includes a first reaction device; a second reaction device, wherein the second reaction device is connected to the first reaction device; a circulation device, wherein the circulation device is respectively connected to the first reaction device and the second reaction device; an acetone storage device; a centrifugal extraction device; a phenethylamine processing device; and a phenethylamine storage device. The first reaction device and the second reaction device have the same structure. A production method of phenethylamine is further provided, which uses transaminase as a catalyst, so that acetophenone and isopropylamine can flow through the transaminase to complete the reaction, the reaction is completed in one step, the production cycle is shortened, and the production cost is reduced.

Disclosed herein are reactions, methods, reagents and compositions that utilize a nonheme iron halogenase enzyme to prepare compounds.

Disclosed herein are reactions, methods, reagents and compositions that utilize a nonheme iron halogenase enzyme to prepare compounds.

The present invention discloses encoding genes of nitrilase mutants and application thereof. The nucleotide sequence of the gene is shown in SEQ ID No.5, and the amino acid sequence of the mutant is shown in SEQ ID No.6. In the present invention, by the protein molecular modification, thermostability of the purified nitrilase LNIT5 is increased by up to 4.5 folds; and by utilizing recombinant E. coli containing the nitrilase mutant to hydrolyze 1-cyanocyclohexylacetonitrile at a high temperature, product tolerance is increased, activity of NIT5-L201F is increased by 20%, and the mutant NITLNIT5-AcN can completely hydrolyze 750 mM 1-cyanocyclohexylacetonitrile within 8 hours and achieve an doubled conversion rate. Therefore, the mutants obtained by the present invention have a good application prospect in efficiently catalyzing 1-cyanocyclohexylacetonitrile to synthesize gabapentin intermediate, 1-cyanocyclohexyl acetic acid.

The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof. Further contemplated are cultured recombinant host cells as well as methods of producing fatty amines by employing these host cells.