The present invention provides methods, compositions, and articles of manufacture useful for the prophylactic and therapeutic amelioration and treatment of gram-positive bacteria, and related conditions. The present invention provides compositions and methods incorporating and utilizing antibiotics represented by Formulae (I)-(IX) or derivatives or variants thereof.

Enzymes and methods are described herein for manufacturing terpenes, including terpenes.

The present invention relates to a process for preparing a composition comprising retinol, an oil and carnosic acid, where the carnosic acid reduces or prevents the oxidation of retinol and/or enhances the oxidative stability of retinol. The present invention also relates to compositions obtainable by the process and formulations containing said compositions, as well as to methods of reducing or preventing oxidation of retinol and/or enhancing the oxidative stability of retinol.

The present invention relates to a process for preparing a composition comprising retinol, an oil and carnosic acid, where the carnosic acid reduces or prevents the oxidation of retinol and/or enhances the oxidative stability of retinol. The present invention also relates to compositions obtainable by the process and formulations containing said compositions, as well as to methods of reducing or preventing oxidation of retinol and/or enhancing the oxidative stability of retinol.

The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

The present invention provides a method for preparing a steviol glycoside and/or steviol comprising reacting glycosidase AOBGL1 and/or AOBGL3 or a variant thereof with a steviol glycoside having at least one monoglucoside bond and/or monoglucosyl ester bond, thereby cleaving said monoglucoside bond and/or monoglucosyl ester bond.

The present invention provides a method for preparing a steviol glycoside and/or steviol comprising reacting glycosidase AOBGL1 and/or AOBGL3 or a variant thereof with a steviol glycoside having at least one monoglucoside bond and/or monoglucosyl ester bond, thereby cleaving said monoglucoside bond and/or monoglucosyl ester bond.