The present disclosure provides use of sativene, and a recombinant biocontrol fungus and a muscardine cadaver and use thereof, belonging to the technical field of genetic engineering. The present disclosure provides use of sativene in insect attraction and/or pest control. The sativene are attractive to insects. A higher concentration of the sativene has a stronger attraction on mosquito and Drosophila larvae; the Drosophila larvae respond to the sativene even at a level as low as 10.sup.?11 g. The sativene also be attractive to Galleria mellonella larvae. The present disclosure further provides a recombinant biocontrol fungus, including a recombinant fungal expression plasmid, where the recombinant fungal expression plasmid is inserted with a pine longifolene synthesis gene. In the present disclosure, the recombinant biocontrol fungus has increased synthetic amounts and volatilized amounts of the longifolene and sativene, resulting in an enhanced attraction to insects.

Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

The present invention discloses a method for rapidly characterizing content variations of triterpenoids in a liquid fermentation process of Antrodia camphorata, belonging to the field of microbial fermentation. According to the method of the present invention, in the liquid fermentation of Antrodia camphorata, an analysis method of rapidly judging the content variations of triterpenoids by rapid on-line or off-line detection and analysis of the content of a volatile aromatic substance -terpineol is utilized to implement automatic control of the fermentation process. Predictive analysis of triterpenoids in the fermentation process based on variations in on-line real-time parameters increases the controllability and production predictability of the fermentation process. This is of great significance for the development and utilization of Antrodia camphorata products having various bioactivities and application thereof in industrial production.

The present invention discloses a method for rapidly characterizing content variations of triterpenoids in a liquid fermentation process of Antrodia camphorata, belonging to the field of microbial fermentation. According to the method of the present invention, in the liquid fermentation of Antrodia camphorata, an analysis method of rapidly judging the content variations of triterpenoids by rapid on-line or off-line detection and analysis of the content of a volatile aromatic substance -terpineol is utilized to implement automatic control of the fermentation process. Predictive analysis of triterpenoids in the fermentation process based on variations in on-line real-time parameters increases the controllability and production predictability of the fermentation process. This is of great significance for the development and utilization of Antrodia camphorata products having various bioactivities and application thereof in industrial production.

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.

This invention provides recombinant cells and methods for producing terpenes and terpenoids by increasing production or accumulation or both of isoprenoid precursors thereof.

This invention provides recombinant cells and methods for producing terpenes and terpenoids by increasing production or accumulation or both of isoprenoid precursors thereof.

The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.

The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.