This document describes biochemical pathways for producing 7-aminoheptanoic acid using a β-ketoacyl synthase or a β-ketothiolase to form an N-acetyl-5-amino-3-oxopentanoyl-CoA intermediate. 7-aminoheptanoic acid can be enzymatically converted to pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol or corresponding salts thereof. This document also describes recombinant microorganisms producing 7-aminoheptanoic acid as well as pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol or corresponding salts thereof.

Provided are a method and a system for culturing cells and harvesting biologics. More particularly process for cell culture by intensified perfusion with continuous harvest and without cell bleeding is provided.

Provided are a method and a system for culturing cells and harvesting biologics. More particularly process for cell culture by intensified perfusion with continuous harvest and without cell bleeding is provided.

Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

Unique carbon dioxide or lignocellulosic substrate is prepared and used to produce biosurfactants, based on different types of microorganism fermenting strains, using carbon dioxide or lignocellulose-based raw materials as the primary feedstock, subsequently utilizing a fermentation process to synthesize different structures of biosurfactants. This is a two-phase reaction where phase-one creates the feedstock for the phase-two reactions. The fermentation broth resulting from the phase-two reaction is the crude biosurfactant; it uses glycolipid or lipopeptide biosurfactant as the main component. The broth is then refined by filtration, then concentrated, and further purified to obtain the pure biosurfactant material. The biosurfactant of the present disclosure can be applied to industries such as petroleum, food or agriculture, daily chemicals, industrial chemicals, environmental protection, and medicine.

The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product.

The present application provides methods and uses of O-oligosaccharyltransferase (O-OTases) for generating vaccines. In particular, the present application provides a method of synthesizing a glycoprotein comprising glycosylation of pilin-like protein ComP using a Pg1L.sub.ComP O-OTase. Uses of glycoproteins synthesized by glycosylating ComP using Pg1L.sub.ComP O-OTase, particularly for the preparation of vaccines and the like, including a vaccine to Streptococcus, is also provided.

Described are engineered cells that include genetic alterations leading to up- or down-regulation of certain genes in the cells for improved production of a recombinant protein, especially one that is not easily expressed at high levels in unaltered cell lines. Also provided are methods of preparing and using such cells.

Described are engineered cells that include genetic alterations leading to up- or down-regulation of certain genes in the cells for improved production of a recombinant protein, especially one that is not easily expressed at high levels in unaltered cell lines. Also provided are methods of preparing and using such cells.

A non-naturally occurring cell comprising an inoperative genomic asparaginase (Aspg) gene and an inoperative glutamine synthetase (Gs) gene, wherein the cell has been transfected with a controllably expressed gene encoding an enzyme having asparaginase activity, a controllably expressed gene encoding an enzyme having glutamine synthetase activity, and a controllably expressed gene encoding a heterologous protein of interest.