Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.

Provided herein are methods for a robust production of isoprenoids via one or more biosynthetic pathways. Also provided herein are nucleic acids, enzymes, expression vectors, and genetically modified host cells for carrying out the subject methods. Also provided herein are fermentation methods for high productivity of isoprenoids from genetically modified host cells.

Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

Disclosed are a recombinant microorganism for producing a hydrophobic material, which is subjected to cell-membrane engineering in order to be imparted with at least one characteristic among an increase in a cell-membrane area, an increase in formation and secretion of an outer membrane vesicle, and an increase in formation of an inner membrane vesicle, and a cell-membrane engineering method for preparation thereof, whereby an insoluble hydrophobic material can be produced with high efficiency, the recombinant microorganism for high-efficiency production of carotenoids or violacein analogues is useful for producing natural pigments, antioxidants, antibiotics, cosmetic additives, anticancer agents, food additives, or nutritional supplements, and the natural pigment production technology developed herein achieves a great increase in production ability. Therefore, the present invention is effective at preparing a recombinant strain for efficient production of a variety of industrially and medically useful metabolites and at establishing an efficient preparation method.

The invention is directed to a culture medium for Haematococcus that contains combustion gases like carbon dioxide, carbon monoxide, and oxides of nitrogen or sulfur and which fixes the carbon, nitrogen or sulfur in these combustion gases into biomass and to methods providing superior biomass yields using this culture medium to culture select species of Haematococcus such as Haematococcus sp. KAU-01.

The present invention provides a method of producing astaxanthin and lysine in recombinant gram-positive bacteria comprising a nucleic acid sequence encoding for a crtZ-protein from F. pelagi and comprises a nucleic acid sequence encoding for a crtW-protein.

The present invention provides a method of producing astaxanthin and lysine in recombinant gram-positive bacteria comprising a nucleic acid sequence encoding for a crtZ-protein from F. pelagi and comprises a nucleic acid sequence encoding for a crtW-protein.

Provided are a microorganism for producing retinol, in which retinol biosynthetic genes are introduced; and a method of producing retinol, the method including a step of culturing the microorganism. The microorganism of the present invention may have an improved ability to produce retinol, and thus it may be efficiently used in producing retinol. Based on the method of producing retinol, the method including the step of culturing the microorganism, the retinol production efficiency may be improved.

Provided are a microorganism for producing retinol, in which retinol biosynthetic genes are introduced; and a method of producing retinol, the method including a step of culturing the microorganism. The microorganism of the present invention may have an improved ability to produce retinol, and thus it may be efficiently used in producing retinol. Based on the method of producing retinol, the method including the step of culturing the microorganism, the retinol production efficiency may be improved.

A novel alga mutant having improved pigment productivity is provided. The mutant can be used to produce carotenoid-based pigments, particularly, xanthophyll by using a small amount of energy, and thus can effectively produce pigment on an industrial level. In addition, the mutant can be used as a source for foods, health functional foods, and medications which contain a pigment. Further, when account is taken of the physiological characteristics of the euryhaline microalgae Dunaliella and the geological characteristics of Korean, the three sides of which are in contact with the sea, sea-water can be used as a culture medium, with the expectation of reducing the cost and leading to the development of related industries.