The present invention relates to the preparation of organic molecules by anaerobic fermentation of biomass, in which the fermentation liquor is aerated prior to the organic molecule recovery step.

The present invention relates to the preparation of organic molecules by anaerobic fermentation of biomass, in which the fermentation liquor is aerated prior to the organic molecule recovery step.

In the combined process for several biorefinery products obtained from a UMC (Undefined Mixed Culture) type of reaction it is possible to obtain biochemicals, energy gases, soil improvement etc. from a MPBU (Multipurpose Biorefinery Unit). The economically beneficial as well as environmentally sustainable results of the arrangement are demonstrated by the integrated process using two reactor systems with zero fiber for the production of lactate (in both the reactors pools 1 and 2). Additionally, mannitol can be produced in one of the reactor pools (number 2). It is possible to a. combine the processes taking into account their biochemical characteristics, b. produce gaseous substances for energy and industrial use, c. obtain organic fertilizers which can be microbiologically upgraded d. improve the adjustability for optimization of the various partial reactivities.

The chemical production occurs in two pools which advantageously are inoculated simultaneously.

In the combined process for several biorefinery products obtained from a UMC (Undefined Mixed Culture) type of reaction it is possible to obtain biochemicals, energy gases, soil improvement etc. from a MPBU (Multipurpose Biorefinery Unit). The economically beneficial as well as environmentally sustainable results of the arrangement are demonstrated by the integrated process using two reactor systems with zero fiber for the production of lactate (in both the reactors pools 1 and 2). Additionally, mannitol can be produced in one of the reactor pools (number 2). It is possible to a. combine the processes taking into account their biochemical characteristics, b. produce gaseous substances for energy and industrial use, c. obtain organic fertilizers which can be microbiologically upgraded d. improve the adjustability for optimization of the various partial reactivities.

The chemical production occurs in two pools which advantageously are inoculated simultaneously.

The present invention relates to an integrated method for methane rich biogas production from anaerobe systems using multiple reactors and various types of on-site selective enriched microbial consortium production. Biomass/organic waste is converted to biogas rich in methane content and minimum or absence of hydrogen sulphide content at reduced hydraulic retention time (HRT). More particularly, the present invention relates to an on-site enrichment of selective microbe with specified biochemical function and its dosage into the respective digester at regular time intervals which improve the biomass conversion through biochemical steps, resulting into maximum production of methane rich biogas in a self-controlled biochemical function inside each bioreactor in a comparatively lesser time intervals.

A method, process, and computer program for determining an order in which a food product is successively cultured with a set of bacterial cultures in a process for producing a fermented food product, each bacterial culture of the set of bacterial cultures including at least one bacterial strain, wherein the set of bacterial cultures comprises a first subset of one or more bacterial cultures comprising one or more unknown bacterial strain, and a second subset of one or more bacterial cultures comprising one or more known bacterial strains, wherein each of the bacterial strains of the second subset is known; wherein the process is carried out with an initial order of the set of bacterial cultures; wherein during culturing with each bacterial culture of the first subset a process sample is collected, wherein a culture sample of a bacterial culture of the second subset is exposed to the collected process samples in order to determine bacteriophage sensitivities of the one or more bacterial cultures of the second subset to bacteriophages present in the collected process sample, wherein an adapted order of the set of bacterial cultures is determined based on the determined bacteriophage sensitivities such as to reduce common bacteriophage sensitivities in successive bacterial cultures.

A method, process, and computer program for determining an order in which a food product is successively cultured with a set of bacterial cultures in a process for producing a fermented food product, each bacterial culture of the set of bacterial cultures including at least one bacterial strain, wherein the set of bacterial cultures comprises a first subset of one or more bacterial cultures comprising one or more unknown bacterial strain, and a second subset of one or more bacterial cultures comprising one or more known bacterial strains, wherein each of the bacterial strains of the second subset is known; wherein the process is carried out with an initial order of the set of bacterial cultures; wherein during culturing with each bacterial culture of the first subset a process sample is collected, wherein a culture sample of a bacterial culture of the second subset is exposed to the collected process samples in order to determine bacteriophage sensitivities of the one or more bacterial cultures of the second subset to bacteriophages present in the collected process sample, wherein an adapted order of the set of bacterial cultures is determined based on the determined bacteriophage sensitivities such as to reduce common bacteriophage sensitivities in successive bacterial cultures.

A method for treating biomass including lignocellulosic polymers. The biomass is treated in a mixture of water with at least one oxidizing agent and steam at a temperature in a range of from about 130° C. to about 220° C. for a period from about 5 seconds to about 10 hours. The pH of the mixture is periodically measured for substantially an entire duration of the treating step. As necessary, based on the measured pH of the mixture, adjusting the pH of the mixture into a range of from about pH 4.5 to about pH 7.5 by adding a base to the mixture.

A method for treating biomass including lignocellulosic polymers. The biomass is treated in a mixture of water with at least one oxidizing agent and steam at a temperature in a range of from about 130° C. to about 220° C. for a period from about 5 seconds to about 10 hours. The pH of the mixture is periodically measured for substantially an entire duration of the treating step. As necessary, based on the measured pH of the mixture, adjusting the pH of the mixture into a range of from about pH 4.5 to about pH 7.5 by adding a base to the mixture.

An integrated process and corresponding apparatus that produces a relatively clean, delignified cellulose product from lignocellulosic biomass. The method includes treating a portion of the delignified cellulose itself as a substrate to produce on-site cellulolytic enzymes, including further treating the remaining delignified cellulose with the resulting cellulolytic enzymes for in situ enzymatic hydrolysis. The process and apparatus are useful to produce fermentable sugars for cost-effective manufacturing of fermentable sugars, fuels, bioproducts and chemicals.