Methods are provided for enhanced production of one or more microbial biopolymers, the methods comprising co-cultivating Stigmatella aurantiaca and Acinetobacter venetianus. In certain embodiments, the one or more biopolymers are emulsan. In certain embodiments, other microbial growth by-products are produced, such as biosurfactants. Microbe-based products produced according to the subject methods are also provided, as well as their uses in, for example, oil and gas recovery, agriculture, and health care.

An integrated process and corresponding apparatus that produces a relatively clean, delignified cellulose product from lignocellulosic biomass. The method includes treating a portion of the delignified cellulose itself as a substrate to produce on-site cellulolytic enzymes, including further treating the remaining delignified cellulose with the resulting cellulolytic enzymes for in situ enzymatic hydrolysis. The process and apparatus are useful to produce fermentable sugars for cost-effective manufacturing of fermentable sugars, fuels, bioproducts and chemicals.

Biological agent for bio-augmentation of an anaerobic digestion reactor, named first anaerobic digestion reactor, comprising a mixture of microorganisms including at least 10% of relative abundance in said mixture, of a unique Cloacimonetes sp. This agent is prepared by enrichment of a biological sample in a separate reactor fed with carbohydrate-rich substrate and oxygenated gas. Said agent comprising this unique Cloacimonetes sp. is able to restore and stabilize the biogas production of an anaerobic digestion reactor after acidosis, in a very short time.

Biological agent for bio-augmentation of an anaerobic digestion reactor, named first anaerobic digestion reactor, comprising a mixture of microorganisms including at least 10% of relative abundance in said mixture, of a unique Cloacimonetes sp. This agent is prepared by enrichment of a biological sample in a separate reactor fed with carbohydrate-rich substrate and oxygenated gas. Said agent comprising this unique Cloacimonetes sp. is able to restore and stabilize the biogas production of an anaerobic digestion reactor after acidosis, in a very short time.

A method of preparing a bacterial composition is disclosed. The method comprises: (a) in vitro co-culturing beneficial bacteria with biofilm-producing bacteria in a growth substrate under conditions that generate a biofilm which comprises the beneficial bacteria and the non-pathogenic bacteria; and (b) isolating the biofilm from the growth substrate.

A method of preparing a bacterial composition is disclosed. The method comprises: (a) in vitro co-culturing beneficial bacteria with biofilm-producing bacteria in a growth substrate under conditions that generate a biofilm which comprises the beneficial bacteria and the non-pathogenic bacteria; and (b) isolating the biofilm from the growth substrate.

The subject invention provides microbe-based products and efficient methods of producing them. In specific embodiments, methods are provided for symbiotic cultivation of Acinetobacter venetianus RAG1 and Bacillus subtilis B1, as well as growth by-products thereof. Methods are also provided for using the subject microbe-based products, for example, in microbially enhanced oil recovery (MEOR).

The subject invention provides microbe-based products and efficient methods of producing them. In specific embodiments, methods are provided for symbiotic cultivation of Acinetobacter venetianus RAG1 and Bacillus subtilis B1, as well as growth by-products thereof. Methods are also provided for using the subject microbe-based products, for example, in microbially enhanced oil recovery (MEOR).

Provided herein are amphoteric glycolipid biosurfactants containing an amphoteric glycolipid biological surface active molecule preparation produced by acid precipitation of culture supernatant produced by sequentially culturing Pseudomonas, Candida, and Neurospora, for instance in a fermentation medium comprising a hydrophilic carbon source and a hydrophobic carbon source. Also described are amphoteric glycolipid biological surface active molecule preparations, and methods of making such preparations. The amphoteric molecules have anionic and cationic groups; example molecules include 17-L-[(2′-O-β-D-glucopyranosyl-β-D-Glucosyl)-O-]-octadecenoic acid amine-6′,6″ diacetate, and 17-L-[(2′-O-β-D-glucopyranosamine-β-D-rhamnosyl)-O-]-octadecenoic acid-6′,6″ diacetate. The amphoteric glycolipid biosurfactant has good compatibility with other types of surfactants, high temperature resistance and salt resistance, and is suitable for use in a variety of liquid systems.

Provided herein are compositions, methods, systems associated with propagation and fermentation, and co-products of biochemical production processes, for example, a DCO co-product resulting from converting oil containing grains into bio chemicals via fermentation in the presence of endogenous esterase. The DCO resulting from the processes exhibits lower metal ion content relative to a DCO obtained in the absence of endogenous fermentation with an esterase such as a lipase. The DCO is useful as a feedstock for the production of renewable diesel.