Ethanol is produced by the simultaneous production of both First and Second generation (1G, 2G) fuel grade ethanol in the same production plant. A First Generation feedstock such as corn is continuously fed to the first generation section and a lignocellulosic feedstock such as corn stover from the 1G corn is supplied to the second generation area Thus, there is a common fermentation area for both the C5 and C6 sugar fermentation. The invention can economically be best implemented in places where there are incentives offered for the use of various feedstocks. Specifically, the invention allows the D3 rin to be maximized in an existing first-generation ethanol plant with the installation of the front end of the 2G equipment.

The present invention relates to a process for the extraction of valuable components from a tannin-rich bark raw-material, by carrying out an alkaline cooking step, followed by acid precipitation to separate the valuable components from the remaining bark pulp. The invention also relates to the use of said process on a bark side stream of a chemical pulping plant, whereby the liquor remaining after the extraction of the present process is returned to a stream of the wood pulping process, typically to a black liquor stream, while the spent pulp can be processed further, e.g. by bleaching to give dissolved pulp.

The present disclosure relates to a physically pretreated biomass composition, which enables chemical treatment in a biomass composition comprising a high concentration of a biomass substrate, and a preparation method thereof.

Through specific physical pretreatment (attrition milling) of herbaceous biomass, the physically pretreated biomass composition has fluidity/flowability at a substrate concentration of biomass of 20% (w/w) (biomass:solvent=1:4) or above, and thus the pretreatment cost may be reduced, and it is very useful in biomass treatment processes.

The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention describes a process for pretreatment of lignocellulosic biomass that comprises the step of contacting a lignocellulosic biomass with an ionic liquid consisting of a phthalic salt of dicholine in the weight ratio from 1:1 to 1:100 of biomass:ionic liquid, said step taking place for a period of time that varies from 0.4 to 48 hours and in a temperature range that varies from 60 to 200° C. Furthermore, the present invention also relates to the use of the pretreated lignocellulosic biomass in an enzymatic hydrolysis process.

The present invention relates to a process for treating lignocellulosic biomass, successively comprising b) a step of pretreatment of the biomass placed beforehand under acidic or neutral pH conditions in a pretreatment reactor (3), to produce an acidic or neutral pretreated must (AM), alternating with b′) a step of pretreatment of the biomass placed beforehand under acidic, neutral or basic conditions, with optional sufficient introduction of a basic aqueous solution (EB) into the pretreatment reactor (3) to produce a basic pretreated must (BM), and then c) a step of enzymatic hydrolysis in a hydrolysis reactor (16) of a mixture of the acidic or neutral pretreated must (AM) obtained from step b) with the basic pretreated must (BM) obtained from step b′).

The invention relates to a process for the preparation of an enzyme composition from cellulosic material.

An advantageous process for pre-treating lignocellulosic biomass to hydrolysable polysaccharide enriched biomass with reduced amount of inhibitors of catalysis comprising steps of: providing a feedstock comprising cellulosic biomass; chopping of cellulosic biomass to have a cellulosic biomass feed material of uniform size; feeding of uniform sized feed material to a horizontal counter current extraction unit; removing excess of water from the feed material obtained from the extraction unit; soaking the extruded feed material obtained from the extraction unit; optionally removing excess of water from the feed material obtained after acid soaking; steam pre-treating the feed material obtained after removing excess of water from the acid soaked feed material; neutralizing the slurry obtained from the steam pre-treating; and optionally saccharifying the neutralized slurry.

The disclosure belongs to the technical field of papermaking technology and waste comprehensive utilization, and specifically relates to a method for preparing biomechanical unbleached pulp from wheat straw and full utilization of by-products thereof. In the present disclosure, wheat straw is used as raw materials for pulping, and treated by hot water, then a trace of KOH is added, the temperature of the wheat straw after heat saturation and softening is adjusted, alkaline compound enzymes is added for biological treatment, and refining of wheat straw is performed at last. The mechanical pulp meets the production requirements for unbleached packaging paper and paper-based materials, meanwhile, the by-products are recycled to prepare a biomass compound fertilizer, turning solid waste into treasures, and realizing a high value full utilization of wheat straw. Thus a good practical application value and a broad application prospect are performed.

The disclosure relates to a method for preparing unbleached biomechanical pulp by hot steam and biological enzyme treatment and full utilization of by-products thereof, and belongs to the technical field of papermaking technology and comprehensive utilization of waste. The present disclosure proposes a method for preparing high-strength unbleached biomechanical pulp, using whole wheat straw as a raw material, by using hot water in coordination with an alkaline biological enzyme to treat whole wheat straw, thereby meeting the requirements for the production of unbleached linerboard and paper-based materials, and recycling by-products thereof to prepare biomass compound fertilizers, which creates wealth from solid waste and realizes the high-value full utilization of wheat straw. The preparation method of the disclosure is simple, green, clean and efficient, and has good practical application value and broad application prospects.