There is provided an apparatus comprising a receptacle to receive a sample. A sample dispenser dispenses the sample into the receptacle and an image capture device captures an image of the sample in the receptacle. Processing circuitry processes the image to determine whether the receptacle contains zero cells, exactly one cell, or more than one cell. In response to the processor determining that the receptacle contains zero cells, the processing circuitry causes the sample dispenser to dispense a further sample into the receptacle.

A cell-trapping device includes a microchannel portion for trapping a plurality of cells with an average diameter of at most 25 μm for high-throughput microinjection of an injectant into the cells. The cell-trapping device includes a microchannel portion having formed therein a cell-trapping area including a plurality of cell-trapping microchannels configured to trap one cell per cell-trapping channel. A method for preparing the cell-trapping device and an apparatus for high-throughput microinjection is also provided. Further provided is a method for injecting an injectant into a plurality of cells. The cell-trapping device, apparatus, and method allow for a rapid and highly reproducible microinjection into small cells with high productivity, high accuracy and a good cell survival rate.

A cell-trapping device includes a microchannel portion for trapping a plurality of cells with an average diameter of at most 25 μm for high-throughput microinjection of an injectant into the cells. The cell-trapping device includes a microchannel portion having formed therein a cell-trapping area including a plurality of cell-trapping microchannels configured to trap one cell per cell-trapping channel. A method for preparing the cell-trapping device and an apparatus for high-throughput microinjection is also provided. Further provided is a method for injecting an injectant into a plurality of cells. The cell-trapping device, apparatus, and method allow for a rapid and highly reproducible microinjection into small cells with high productivity, high accuracy and a good cell survival rate.

Disclosed is a method for controlling the carbon feed to a fed-batch fermenter based on the disturbance of the pH signal following the addition or a limiting substrate.

Disclosed is a method for controlling the carbon feed to a fed-batch fermenter based on the disturbance of the pH signal following the addition or a limiting substrate.

The present disclosure relates to settling devices for separating particles from a bulk fluid with applications in numerous fields. The particle settling devices of the present disclosure may include a stack of truncoconical cones that may be arranged in opposite orientation, apex to base. Other embodiments include several concentric vertical tubes attached to conical surfaces at the bottom, with inclined settling strips attached to the vertical tubes in annular regions between the tubes. These devices are useful for separating small (millimeter or micron sized) particles from a bulk fluid with applications in numerous fields, such as biological (microbial, mammalian, plant, insect or algal) cell cultures, solid catalyst particle separation from a liquid or gas and waste water treatment.

The present disclosure relates to settling devices for separating particles from a bulk fluid with applications in numerous fields. The particle settling devices of the present disclosure may include a stack of truncoconical cones that may be arranged in opposite orientation, apex to base. Other embodiments include several concentric vertical tubes attached to conical surfaces at the bottom, with inclined settling strips attached to the vertical tubes in annular regions between the tubes. These devices are useful for separating small (millimeter or micron sized) particles from a bulk fluid with applications in numerous fields, such as biological (microbial, mammalian, plant, insect or algal) cell cultures, solid catalyst particle separation from a liquid or gas and waste water treatment.

Disclosed herein are systems, devices and methods for processing tissue, such as autologous tissue. Implementations of a Stromal Vascular Fraction (SVF) system are described that can isolate and wash harvested cells contained within various tissues, such as isolate and wash stem cells from fat tissue. The SVF system can minimize the handling and transferring of tissue and fluids, including minimizing the number of human interventions and manipulations required throughout processing. The SVF system can ensure sterility of processed tissue and harvested cells, as well as significantly reduce cost and time associated with the processing.

Disclosed herein are systems, devices and methods for processing tissue, such as autologous tissue. Implementations of a Stromal Vascular Fraction (SVF) system are described that can isolate and wash harvested cells contained within various tissues, such as isolate and wash stem cells from fat tissue. The SVF system can minimize the handling and transferring of tissue and fluids, including minimizing the number of human interventions and manipulations required throughout processing. The SVF system can ensure sterility of processed tissue and harvested cells, as well as significantly reduce cost and time associated with the processing.

A method and an apparatus for producing biogas from organic matter including a container (1) which is charged with fermentation substrate by a delivery system (13). At least one stirring mechanism (2) is arranged in the container. The feedback value of at least one measurable variable is detected and transmitted to a control unit (4). A reference variable is also provided to the control unit. The control unit calculates the deviation of the feedback value from the reference value, and actuating variables which modify the power input of the stirring mechanism and/or the composition of the container contents and/or the flow behavior of the container contents are adjusted as a function of the deviation.